ArticleNature protocols2012
Derivation and propagation of human embryonic stem cell lines from frozen embryos in an animal product-free environment.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 74 citations in OpenAlex.
- A simple and standardized method supports efficient derivation of clinical-grade human embryonic stem cells under feeder- and xeno-free conditions.Stem cell research & therapy · 2025Article
- Animal product-free formation and cultivation of three-dimensional primary hepatocyte spheroids.Drug metabolism and disposition: the biological fate of chemicals · 2025Article
- Differential Gene Regulation of the Human Blastocyst Trophectoderm and Inner Cell Mass by Progesterone.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Efficient derivation and banking of clinical-grade human embryonic stem cell lines in accordance with Japanese regulations.Regenerative therapy · 2022Article
- Comparison of human isogeneic Wharton's jelly MSCs and iPSC-derived MSCs reveals differentiation-dependent metabolic responses to IFNG stimulation.Cell death & disease · 2019Article
- Prospects for the Use of Induced Pluripotent Stem Cells in Animal Conservation and Environmental Protection.Stem cells translational medicine · 2019Article
- High quality clinical grade human embryonic stem cell lines derived from fresh discarded embryos.Stem cell research & therapy · 2017Article
- Induced Pluripotent Stem Cell Differentiation and Three-Dimensional Tissue Formation Attenuate Clonal Epigenetic Differences in Trichohyalin.Stem cells and development · 2016Article
- Generation of KCL027 research grade human embryonic stem cell line carrying a mutation in the HTT gene.Stem cell research · 2016Article
- Generation of KCL033 clinical grade human embryonic stem cell line.Stem cell research · 2016Article
- Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene.Stem cell research · 2016Article
- Generation of KCL036 research grade human embryonic stem cell line carrying a mutation in the HTT gene.Stem cell research · 2016Article
- Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene.Stem cell research · 2016Article
- Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene.Stem cell research · 2016Article
- Generation of KCL024 research grade human embryonic stem cell line carrying a mutation in NF1 gene.Stem cell research · 2016Article
- Generation of KCL026 research grade human embryonic stem cell line carrying a mutation in SMN1 gene.Stem cell research · 2016Article
- Generation of KCL012 research grade human embryonic stem cell line carrying a mutation in the HTT gene.Stem cell research · 2016Article
- Generation of KCL025 research grade human embryonic stem cell line carrying a mutation in NF1 gene.Stem cell research · 2016Article
- Generation of KCL017 research grade human embryonic stem cell line carrying a mutation in VHL gene.Stem cell research · 2016Article
- Generation of KCL028 research grade human embryonic stem cell line carrying a mutation in the HTT gene.Stem cell research · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
The protocols described here are comprehensive instructions for deriving human embryonic stem (hES) cell lines in xeno-free conditions from cryopreserved embryos. Details are included for propagation, cryopreservation and characterization. Initial derivation is on feeder cells and is followed by adaptation to a feeder-free environment; competent technicians can perform these simplified methods easily. From derivation to cryopreservation of fully characterized initial stocks takes 3-4 months. These protocols served as the basis for standard operating procedures (SOPs), with both operational and technical components, that we set to meet good manufacturing practice (GMP) and UK regulatory body requirements for derivation of clinical-grade cells. As such, these SOPs are currently used in our current GMP compliant facility to derive hES cell lines ab initio, in an animal product-free environment; these lines are suitable for research and potentially for clinical use in cell therapy. So far, we have derived eight clinical-grade lines, which will be freely available to the scientific community after submission/accession to the UK Stem Cell Bank.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.