ArticleGenome research2012
Drosophila CTCF tandemly aligns with other insulator proteins at the borders of H3K27me3 domains.
Article in Genome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
96 citing papers in PubMed, 132 citations in OpenAlex.
- Single-molecule chromatin tracing reveals a diversity of megabase heterochromatin domains.Nature structural & molecular biology · 2026Article
- Drosophila Rumpelstiltskin regulates Polycomb cis and trans interactions and Homie barrier activity.Epigenetics & chromatin · 2026Article
- A wavelet-based approach generates quantitative, scale-free and hierarchical descriptions of 3D genome structures and new biological insights.PLoS computational biology · 2026Article
- Precise control of chromatin loop extrusion enhances sustainable green revolution yield in rice.Nature genetics · 2025Article
- The homie insulator has sub-elements with different insulating and long-range pairing properties.Genetics · 2025Article
- ThebioRxiv : the preprint server for biology · 2025Article
- Multiscale 3D genome rewiring during PTF1A-mediated somatic cell reprogramming into neural stem cells.Communications biology · 2024Article
- Members of an array of zinc-finger proteins specify distinct Hox chromatin boundaries.Molecular cell · 2024Article
- Chromatin insulator mechanisms ensure accurate gene expression by controlling overall 3D genome organization.Current opinion in genetics & development · 2024Review
- Metabolic regulation of CTCF expression and chromatin association dictates starvation response in mice and flies.iScience · 2023Article
- Low-affinity CTCF binding drives transcriptional regulation whereas high-affinity binding encompasses architectural functions.iScience · 2023Article
- CTCF, BEAF-32, and CP190 are not required for the establishment of TADs in earlyScience advances · 2023Article
- Topological screen identifies hundreds of Cp190- and CTCF-dependentScience advances · 2023Article
- Chromatin accessibility-based characterisation of brain gene regulatory networks in three distinct honey bee polyphenisms.Nucleic acids research · 2022Article
- Lint-O cooperates with L(3)mbt in target gene suppression to maintain homeostasis in fly ovary and brain.EMBO reports · 2022Article
- Review
- CRISPR and biochemical screens identify MAZ as a cofactor in CTCF-mediated insulation at Hox clusters.Nature genetics · 2022Article
- Single-nucleus RNA-sequencing in pre-cellularization Drosophila melanogaster embryos.PloS one · 2022Article
- Three classes of epigenomic regulators converge to hyperactivate the essential maternal gene deadhead within a heterochromatin mini-domain.PLoS genetics · 2022Article
- An explainable artificial intelligence approach for decoding the enhancer histone modifications code and identification of novel enhancers in Drosophila.Genome biology · 2021Article
36 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Several multiprotein DNA complexes capable of insulator activity have been identified in Drosophila melanogaster, yet only CTCF, a highly conserved zinc finger protein, and the transcription factor TFIIIC have been shown to function in mammals. CTCF is involved in diverse nuclear activities, and recent studies suggest that the proteins with which it associates and the DNA sequences that it targets may underlie these various roles. Here we show that the Drosophila homolog of CTCF (dCTCF) aligns in the genome with other Drosophila insulator proteins such as Suppressor of Hairy wing [SU(HW)] and Boundary Element Associated Factor of 32 kDa (BEAF-32) at the borders of H3K27me3 domains, which are also enriched for associated insulator proteins and additional cofactors. RNAi depletion of dCTCF and combinatorial knockdown of gene expression for other Drosophila insulator proteins leads to a reduction in H3K27me3 levels within repressed domains, suggesting that insulators are important for the maintenance of appropriate repressive chromatin structure in Polycomb (Pc) domains. These results shed new insights into the roles of insulators in chromatin domain organization and support recent models suggesting that insulators underlie interactions important for Pc-mediated repression. We reveal an important relationship between dCTCF and other Drosophila insulator proteins and speculate that vertebrate CTCF may also align with other nuclear proteins to accomplish similar functions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.