ArticleCell death and differentiation2012
Histone demethylase Kdm4b functions as a co-factor of C/EBPβ to promote mitotic clonal expansion during differentiation of 3T3-L1 preadipocytes.
Article in Cell death and differentiation, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 65 citations in OpenAlex.
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- Inhibitory Effect of Isopanduratin A on Adipogenesis: A Study of Possible Mechanisms.Foods (Basel, Switzerland) · 2023Article
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- Immature ginger reduces triglyceride accumulation by downregulating Acyl CoA carboxylase and phosphoenolpyruvate carboxykinase-1 genes in 3T3-L1 adipocytes.Food & nutrition research · 2023Article
- Mechanism of the fungal-like particles in the inhibition of adipogenesis in 3T3-L1 adipocytes.Scientific reports · 2021Article
- HMGB2 orchestrates mitotic clonal expansion by binding to the promoter of C/EBPβ to facilitate adipogenesis.Cell death & disease · 2021Article
- MYOD1 inhibits avian adipocyte differentiation via miRNA-206/KLF4 axis.Journal of animal science and biotechnology · 2021Article
- The Diverse Roles of Histone Demethylase KDM4B in Normal and Cancer Development and Progression.Frontiers in cell and developmental biology · 2021Review
- Lifestyle intervention in individuals with impaired glucose regulation affects Caveolin-1 expression and DNA methylation.Adipocyte · 2020Article
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- Anti-Obesity Effects of Tanshinone I fromNutrients · 2020Article
- Molecular Mechanisms of Adipogenesis: The Anti-adipogenic Role of AMP-Activated Protein Kinase.Frontiers in molecular biosciences · 2020Review
- Epigenetic Regulation of Adipogenic Differentiation by Histone Lysine Demethylation.International journal of molecular sciences · 2019Review
- Histone demethylase KDM5A is transactivated by the transcription factor C/EBPβ and promotes preadipocyte differentiation by inhibiting Wnt/β-catenin signaling.The Journal of biological chemistry · 2019Article
- Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC.Oncogene · 2019Article
- Lipid Accumulation and Chronic Kidney Disease.Nutrients · 2019Review
- KDM4B: A Nail for Every Hammer?Genes · 2019Review
- Histone H3K9 demethylase JMJD2B induces hepatic steatosis through upregulation of PPARγ2.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CCAAT/enhancer-binding protein (C/EBP) β is required for both mitotic clonal expansion (MCE) and terminal adipocyte differentiation of 3T3-L1 preadipocytes. Although the role of C/EBPβ in terminal adipocyte differentiation is well defined, its mechanism of action during MCE is not. In this report, histone demethylase Kdm4b, as well as cell cycle genes Cdc45l (cell division cycle 45 homolog), Mcm3 (mini-chromosome maintenance complex component 3), Gins1 (GINS complex subunit 1) and Cdc25c (cell division cycle 25 homolog c), were identified as potential C/EBPβ target genes during MCE by utilizing promoter-wide chromatin immunoprecipitation (ChIP)-on-chip analysis combined with gene expression microarrays. The expression of Kdm4b is induced during MCE and its induction is dependent on C/EBPβ. ChIP, Electrophoretic Mobility Shift Assay (EMSA) and luciferase assay confirmed that the promoter of Kdm4b is bound and activated by C/EBPβ. Knockdown of Kdm4b impaired MCE. Furthermore, Kdm4b interacted with C/EBPβ and was recruited to the promoters of C/EBPβ-regulated cell cycle genes, including Cdc45l, Mcm3, Gins1, and Cdc25c, demethylated H3K9me3 and activated their transcription. These findings suggest a novel feed forward mechanism involving a DNA binding transcription factor (C/EBPβ) and a chromatin regulator (Kdm4b) in the regulation of MCE by controlling cell cycle gene expression.
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