ArticleScience (New York, N.Y.)2012
Deformations within moving kinetochores reveal different sites of active and passive force generation.
Article in Science (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
64 citing papers in PubMed.
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- An optimum rate of microtubule flux for error correction in metaphase spindle.Life science alliance · 2026Article
- A Computational Pipeline for Quantifying Kinetochore Morphological Changes in Live Cells.bioRxiv : the preprint server for biology · 2026Article
- Mechanical states of a motor protein in the spindle.Current biology : CB · 2026Article
- Mammalian metaphase kinetochores are elastic and require condensin for robust structure and function.bioRxiv : the preprint server for biology · 2025Article
- Mechanism of spindle stability and poleward flux regulating spindle length during the metaphase.iScience · 2025Article
- Ndc80 complex, a conserved coupler for kinetochore-microtubule motility, is a sliding molecular clutch.Science advances · 2025Article
- Optimal strategies for correcting merotelic chromosome attachments in anaphase.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Kinetochores grip microtubules with directionally asymmetric strength.The Journal of cell biology · 2025Article
- Semi-automated 3D fluorescence speckle analyzer (3D-Speckler) for microscope calibration and nanoscale measurement.The Journal of cell biology · 2023Article
- The Astrin-SKAP complex reduces friction at the kinetochore-microtubule interface.Current biology : CB · 2022Article
- Tension can directly suppress Aurora B kinase-triggered release of kinetochore-microtubule attachments.Nature communications · 2022Article
- Ultrafast Force-Clamp Spectroscopy of Microtubule-Binding Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Kre28-Spc105 interaction is essential for Spc105 loading at the kinetochore.Open biology · 2022Article
- Counteraction between Astrin-PP1 and Cyclin-B-CDK1 pathways protects chromosome-microtubule attachments independent of biorientation.Nature communications · 2021Article
- Aurora B Tension Sensing Mechanisms in the Kinetochore Ensure Accurate Chromosome Segregation.International journal of molecular sciences · 2021Review
- Shake It Off: The Elimination of Erroneous Kinetochore-Microtubule Attachments and Chromosome Oscillation.International journal of molecular sciences · 2021Review
- Individual kinetochore-fibers locally dissipate force to maintain robust mammalian spindle structure.The Journal of cell biology · 2020Article
- Cell stretching is amplified by active actin remodelling to deform and recruit proteins in mechanosensitive structures.Nature cell biology · 2020Article
- Effects of malleable kinetochore morphology on measurements of intrakinetochore tension.Open biology · 2020Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Kinetochores mediate chromosome segregation at mitosis. They are thought to contain both active, force-producing and passive, frictional interfaces with microtubules whose relative locations have been unclear. We inferred mechanical deformation within single kinetochores during metaphase oscillations by measuring average separations between fluorescently labeled kinetochore subunits in living cells undergoing mitosis. Inter-subunit distances were shorter in kinetochores moving toward poles than in those moving away. Inter-subunit separation decreased abruptly when kinetochores switched to poleward movement and decreased further when pulling force increased, suggesting that active force generation during poleward movement compresses kinetochores. The data revealed an active force-generating interface within kinetochores and a separate passive frictional interface located at least 20 nanometers away poleward. Together, these interfaces allow persistent attachment with intermittent active force generation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.