ArticleClinica chimica acta; international journal of clinical chemistry2012
Validation of a multiplex immunoassay for serum angiogenic factors as biomarkers for aggressive prostate cancer.
Article in Clinica chimica acta; international journal of clinical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- The Latest Developments in Using Proteomic Biomarkers from Urine and Serum for Non-Invasive Disease Diagnosis and Prognosis.Biomarker insights · 2023Review
- Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays.Biomedicines · 2021Article
- A panel of selected serum protein biomarkers for the detection of aggressive prostate cancer.Theranostics · 2021Article
- Prognostic value of plasminogen activator inhibitor-1 in biomarker exploration using multiplex immunoassay in patients with metastatic renal cell carcinoma treated with axitinib.Health science reports · 2020Article
- Article
- A pilot study to profile salivary angiogenic factors to detect head and neck cancers.BMC cancer · 2018Article
- Development of a reliable assay to measure glypican-1 in plasma and serum reveals circulating glypican-1 as a novel prostate cancer biomarker.Oncotarget · 2018Article
- Diagnostic Value of Serum Angiogenesis Markers in Ovarian Cancer Using Multiplex Immunoassay.International journal of molecular sciences · 2017Article
- Translating pharmacodynamic biomarkers from bench to bedside: analytical validation and fit-for-purpose studies to qualify multiplex immunofluorescent assays for use on clinical core biopsy specimens.Seminars in oncology · 2016Review
- Effects of definitive chemoradiation on circulating immunologic angiogenic cytokines in head and neck cancer patients.Journal for immunotherapy of cancer · 2016Article
- Recommendations for Use and Fit-for-Purpose Validation of Biomarker Multiplex Ligand Binding Assays in Drug Development.The AAPS journal · 2016Review
- A microfluidic multiplex proteomic immunoassay device for translational research.Clinical proteomics · 2015Article
- A novel multiplexed immunoassay identifies CEA, IL-8 and prolactin as prospective markers for Dukes' stages A-D colorectal cancers.Clinical proteomics · 2015Article
- Effects of some natural immunomodulatory compounds in combination with thalidomide on survival rate and tumor size in fibrosarcoma-bearing mice.Advanced pharmaceutical bulletin · 2014Article
- Validation of a multiplex electrochemiluminescent immunoassay platform in human and mouse samples.Journal of immunological methods · 2014Article
- Biomarkers of reactive resistance and early disease progression during chemotherapy plus bevacizumab treatment for colorectal carcinoma.Oncotarget · 2014Article
- Follistatin as potential therapeutic target in prostate cancer.Targeted oncology · 2013Review
- Analysis of serum protein glycosylation by a differential lectin immunosorbant assay (dLISA).Clinical proteomics · 2013Article
- A Multiplex Microsphere-Based Immunoassay Increases the Sensitivity of SIV-Specific Antibody Detection in Serum Samples and Mucosal Specimens Collected from Rhesus Macaques Infected with SIVmac239.BioResearch open access · 2013Article
- Integrated analyses of proteins and their glycans in a magnetic bead-based multiplex assay format.Clinical chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAssays used for discovery of biomarkers should be robust and high-throughput, capable of analyzing a sufficiently large number of samples over a sufficiently long period of time with good precision.
methodsWe evaluated the analytical performance of the Bio-Plex Pro™ Human Cancer Biomarker Panel 1, a 16-plex multiplex immunoassay, in serum for composite profiling of angiogenic factors. Because prostate cancer progression and metastasis are pathological events closely linked to angiogenesis, serum angiogenic factors are ideal candidates as prognostic biomarkers.
resultsOur 5-day evaluation indicated that all 16 assays in the panel had good reproducibility (total precisions over 5 independent plates in 5 days of <20%), adequate sensitivity (LOQs of majority of the assays less than 100 pg/ml), and wide dynamic ranges (linearity of majority of the assays spanning across 3 logs in concentrations).
conclusionsApplying the panel to sera from prostate cancer patients with Gleason scores of 6, 7, 8-10, tumor stages that correlated with clinical outcome, we identified that the levels of sTIE-2, a soluble form of the transmembrane tyrosine kinase receptor for angiopoietins, were increased in patients with Gleason score of 8-10. Future studies are necessary to determine whether sTIE-2 could be used as a prognostic biomarker for identifying aggressive prostate cancer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.