ArticleMalaria journal2012
Comparison of the absolute and relative efficiencies of electroporation-based transfection protocols for Plasmodium falciparum.
Article in Malaria journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Profiling 26S Proteasome Activity ofInternational journal of molecular sciences · 2026Article
- Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management.Journal of biological engineering · 2025Article
- Optimized plasmid loading of human erythrocytes for Plasmodium falciparum DNA transfections.International journal for parasitology · 2024Article
- Sequence elements within the PEXEL motif and its downstream region modulate PTEX-dependent protein export in Plasmodium falciparum.Traffic (Copenhagen, Denmark) · 2024Article
- Mutation in the 26S proteasome regulatory subunitFrontiers in cellular and infection microbiology · 2024Article
- Establishment of a stable transfection and gene targeting system inFrontiers in cellular and infection microbiology · 2023Article
- Article
- Synchronisation of Plasmodium falciparum and P. knowlesi In Vitro Cultures Using a Highly Specific Protein Kinase Inhibitor.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Fast-Track Phenotypic Characterization of Plasmodium falciparum Vaccine Antigens through Lyse-Reseal Erythrocytes Mediated Delivery (LyRED) of RNA Interference for Targeted Translational Repression.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Improved Plasmodium falciparum dilution cloning through efficient quantification of parasite numbers and c-SNARF detection.Malaria journal · 2021Article
- The Transcription Factor PfAP2-O Influences Virulence Gene Transcription and Sexual Development inFrontiers in cellular and infection microbiology · 2021Article
- Article
- A 4-cyano-3-methylisoquinoline inhibitor of Plasmodium falciparum growth targets the sodium efflux pump PfATP4.Scientific reports · 2019Article
- Melatonin activates FIS1, DYN1, and DYN2 Plasmodium falciparum related-genes for mitochondria fission: Mitoemerald-GFP as a tool to visualize mitochondria structure.Journal of pineal research · 2019Article
- Double-stranded RNA reduces growth rates of the gut parasite Crithidia mellificae.Parasitology research · 2019Article
- Heterologous expression inFEBS open bio · 2018Article
- Independent regulation of Plasmodium falciparum rif gene promoters.Scientific reports · 2018Article
- Knockdown of the translocon protein EXP2 in Plasmodium falciparum reduces growth and protein export.PloS one · 2018Article
- Comparison of two methods for transformation of Plasmodium knowlesi: Direct schizont electroporation and spontaneous plasmid uptake from plasmid-loaded red blood cells.Molecular and biochemical parasitology · 2017Article
- The cytosolic glyoxalases ofMicrobial cell (Graz, Austria) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
backgroundSeveral electroporation protocols exist to transfect exogenous DNA into Plasmodium falciparum. To date, however, only a subjective analysis of their relative efficiencies has been reported.
methodsA time-course of luciferase reporter expression is used to provide an objective quantitative analysis of the absolute efficiency of three electroporation techniques; direct electroporation of ring stage infected erythrocytes, preloading of erythrocytes and a novel "double-tap" protocol that combines both approaches.
resultsPreloading of erythrocytes shows a mean efficiency of 9.59 x 10(-6), some 5-180 fold more efficient than matched experiments utilizing the "double-tap" and direct electroporation of ring stage infected erythrocytes alone, respectively.
conclusionEvidence presented here provides the first quantitative assessment of both the absolute and relative efficiencies of a key molecular tool used to study the biology and pathogenesis of this important human pathogen.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.