ArticleThe Biochemical journal2012
Erythrocyte storage increases rates of NO and nitrite scavenging: implications for transfusion-related toxicity.
Article in The Biochemical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Transfusion of red blood cells stored for shorter versus longer duration for all conditions.The Cochrane database of systematic reviews · 2018Pooled it
- Impact of Point-of-care Allogeneic Red Blood Cell Washing on Markers of Transfusion-related Respiratory Complications: A Phase II Randomized Clinical Trial.Anesthesiology · 2025Trial
- Oxidation Reduction Potential (ORP) is Predictive of Complications Following Pediatric Cardiac Surgery.Pediatric cardiology · 2018Trial
- Trial
- Effects of Aged Stored Autologous Red Blood Cells on Human Endothelial Function.American journal of respiratory and critical care medicine · 2015Trial
- Effects of storage-aged red blood cell transfusions on endothelial function in hospitalized patients.Transfusion · 2015Trial
- Red blood cell transfusion in brain injury: Is it solely a matter of hemoglobin threshold?Critical care (London, England) · 2024Article
- Threshold hemoglobin level for delayed cerebral ischemia: A single-center retrospective analysis.Heliyon · 2024Article
- Different-sized extracellular vesicles derived from stored red blood cells package diverse cargoes and cause distinct cellular effects.Transfusion · 2023Article
- Comparing two extracellular additives to facilitate extended storage of red blood cells in a supercooled state.Frontiers in physiology · 2023Article
- Hemoglobin Oxidation Reactions in Stored Blood.Antioxidants (Basel, Switzerland) · 2022Article
- Do We Store Packed Red Blood Cells under "Quasi-Diabetic" Conditions?Biomolecules · 2021Review
- Red blood cell storage lesion: causes and potential clinical consequences.Blood transfusion = Trasfusione del sangue · 2019Review
- Article
- Non-invasive analysis of stored red blood cells using diffuse resonance Raman spectroscopy.The Analyst · 2018Article
- Characterization of Storage-Induced Red Blood Cell Hemolysis Using Raman Spectroscopy.Laboratory medicine · 2018Article
- Role of heme in lung bacterial infection after trauma hemorrhage and stored red blood cell transfusion: A preclinical experimental study.PLoS medicine · 2018Article
- Influence of red blood cell-derived microparticles upon vasoregulation.Blood transfusion = Trasfusione del sangue · 2017Review
- Physiologic Impact of Circulating RBC Microparticles upon Blood-Vascular Interactions.Frontiers in physiology · 2017Review
- Red blood cell transfusion and skeletal muscle tissue oxygenation in anaemic haematologic outpatients.Radiology and oncology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
Storage of erythrocytes in blood banks is associated with biochemical and morphological changes to RBCs (red blood cells). It has been suggested that these changes have potential negative clinical effects characterized by inflammation and microcirculatory dysfunction which add to other transfusion-related toxicities. However, the mechanisms linking RBC storage and toxicity remain unclear. In the present study we tested the hypothesis that storage of leucodepleted RBCs results in cells that inhibit NO (nitric oxide) signalling more so than younger cells. Using competition kinetic analyses and protocols that minimized contributions from haemolysis or microparticles, our data indicate that the consumption rates of NO increased ~40-fold and NO-dependent vasodilation was inhibited 2-4-fold comparing 42-day-old with 0-day-old RBCs. These results are probably due to the formation of smaller RBCs with increased surface area: volume as a consequence of membrane loss during storage. The potential for older RBCs to affect NO formation via deoxygenated RBC-mediated nitrite reduction was also tested. RBC storage did not affect deoxygenated RBC-dependent stimulation of nitrite-induced vasodilation. However, stored RBCs did increase the rates of nitrite oxidation to nitrate in vitro. Significant loss of whole-blood nitrite was also observed in stable trauma patients after transfusion with 1 RBC unit, with the decrease in nitrite occurring after transfusion with RBCs stored for >25 days, but not with younger RBCs. Collectively, these data suggest that increased rates of reactions between intact RBCs and NO and nitrite may contribute to mechanisms that lead to storage-lesion-related transfusion risk.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.