ArticlePloS one2012
Etiologic diagnosis of lower respiratory tract bacterial infections using sputum samples and quantitative loop-mediated isothermal amplification.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00567827 (Identification of Bacterial Species and Their Antibiotic-Resistant Spectrum in Sputum Specimen From the Patients With Pneumonia Via Nuclear Acid Detection Assay), which is not on this map. Cited by 20 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Identification of Bacterial Species and Their Antibiotic-Resistant Spectrum in Sputum Specimen From the Patients With Pneumonia Via Nuclear Acid Detection Assay
Who cites it
20 citing papers in PubMed.
- Epidemiological characteristics of bacterial lower respiratory tract infections in Jingzhou, Central China, 2020-2024.Frontiers in cellular and infection microbiology · 2026Article
- Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia.Frontiers in microbiology · 2026Article
- Assessment of a rapid diagnostic test based on loop-mediated isothermal amplification (LAMP) to identify the most frequent pathogens causing hospital-acquired pneumonia.Frontiers in cellular and infection microbiology · 2025Article
- Comparison of the bacterial microbiome in the pharynx and nasal cavity of persistent, intermittent carriers and non-carriers ofJournal of medical microbiology · 2024Article
- Epidemiology and Diagnostic Accuracy of Respiratory Pathogens in Pediatric Populations: Insights From Global Studies.Cureus · 2024Review
- Lysophosphatidylcholine acyltransferase level predicts the severity and prognosis of patients with community-acquired pneumonia: a prospective multicenter study.Frontiers in immunology · 2023Observational
- Enhanced DNA and RNA pathogen detection via metagenomic sequencing in patients with pneumonia.Journal of translational medicine · 2022Article
- Development of Loop-Mediated Isothermal Amplification Rapid Diagnostic Assays for the Detection ofFrontiers in molecular biosciences · 2021Article
- Assessment of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Pathogenic Bacteria from Respiratory Samples in Patients with Hospital-Acquired Pneumonia.Microorganisms · 2020Article
- Evaluation of bronchoalveolar lavage fluid combined with the loop-mediated isothermal amplification assay in lower respiratory tract infections.American journal of translational research · 2020Article
- Smartphone-based pathogen diagnosis in urinary sepsis patients.EBioMedicine · 2018Article
- Bacterial pathogens were detected from human exhaled breath using a novel protocol.Journal of aerosol science · 2018Article
- Rhinovirus is associated with severe adult community-acquired pneumonia in China.Journal of thoracic disease · 2017Article
- Development of a bead-based suspension array for the detection of pathogens in acute respiratory tract infections.Experimental biology and medicine (Maywood, N.J.) · 2016Article
- A Pilot Study of Quantitative Loop-mediated Isothermal Amplification-guided Target Therapies for Hospital-acquired Pneumonia.Chinese medical journal · 2016Article
- A preliminary study on the potential ofJournal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2016Article
- Application of loop-mediated isothermal amplification (LAMP) assay for the rapid diagnosis of pathogenic bacteria in clinical sputum specimens of acute exacerbation of COPD (AECOPD).International journal of clinical and experimental medicine · 2015Article
- A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods.Biomolecular detection and quantification · 2014Article
- Differences in distribution and drug sensitivity of pathogens in lower respiratory tract infections between general wards and RICU.Journal of thoracic disease · 2014Article
- Human pharyngeal microbiome may play a protective role in respiratory tract infections.Genomics, proteomics & bioinformatics · 2014Article
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Authors and funding
36 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledEtiologic diagnoses of lower respiratory tract infections (LRTI) have been relying primarily on bacterial cultures that often fail to return useful results in time. Although DNA-based assays are more sensitive than bacterial cultures in detecting pathogens, the molecular results are often inconsistent and challenged by doubts on false positives, such as those due to system- and environment-derived contaminations. Here we report a nationwide cohort study on 2986 suspected LRTI patients across P. R. China. We compared the performance of a DNA-based assay qLAMP (quantitative Loop-mediated isothermal AMPlification) with that of standard bacterial cultures in detecting a panel of eight common respiratory bacterial pathogens from sputum samples. Our qLAMP assay detects the panel of pathogens in 1047(69.28%) patients from 1533 qualified patients at the end. We found that the bacterial titer quantified based on qLAMP is a predictor of probability that the bacterium in the sample can be detected in culture assay. The relatedness of the two assays fits a logistic regression curve. We used a piecewise linear function to define breakpoints where latent pathogen abruptly change its competitive relationship with others in the panel. These breakpoints, where pathogens start to propagate abnormally, are used as cutoffs to eliminate the influence of contaminations from normal flora. With help of the cutoffs derived from statistical analysis, we are able to identify causative pathogens in 750 (48.92%) patients from qualified patients. In conclusion, qLAMP is a reliable method in quantifying bacterial titer. Despite the fact that there are always latent bacteria contaminated in sputum samples, we can identify causative pathogens based on cutoffs derived from statistical analysis of competitive relationship.
trial registrationClinicalTrials.gov NCT00567827.
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