ArticlePloS one2012
Pleiotropic effects of deubiquitinating enzyme Ubp5 on growth and pathogenesis of Cryptococcus neoformans.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Deubiquitinase Ubp5 is essential for pulmonary immune evasion and hematogenous dissemination ofMycology · 2025Article
- Cryptococcus neoformans requires the TVF1 gene for thermotolerance and virulence.Medical mycology · 2023Article
- Multiple F-Box Proteins Collectively Regulate Cell Development and Pathogenesis in the Human PathogenJournal of fungi (Basel, Switzerland) · 2022Article
- Review
- The Deubiquitinating EnzymeFrontiers in fungal biology · 2022Article
- Article
- An improved staining method of cell cycle analysis with Sybr Green I for fungi:Cell cycle (Georgetown, Tex.) · 2021Article
- Ovarian Tumor Domain-Containing Proteases-Deubiquitylation Enzyme GeneFrontiers in microbiology · 2021Article
- Pleiotropy and epistasis within and between signaling pathways defines the genetic architecture of fungal virulence.PLoS genetics · 2021Article
- More Than Just Cleaning: Ubiquitin-Mediated Proteolysis in Fungal Pathogenesis.Frontiers in cellular and infection microbiology · 2021Review
- The putative deubiquitinating enzyme MoUbp4 is required for infection-related morphogenesis and pathogenicity in the rice blast fungus Magnaporthe oryzae.Current genetics · 2020Article
- Ribosomal Protein L40e Fused With a Ubiquitin Moiety Is Essential for the Vegetative Growth, Morphological Homeostasis, Cell Cycle Progression, and Pathogenicity ofFrontiers in microbiology · 2020Article
- Differences in Sirtuin Regulation in Response to Calorie Restriction in Cryptococcus neoformans.Journal of fungi (Basel, Switzerland) · 2018Article
- A Deubiquitinating Enzyme Ubp14 Is Required for Development, Stress Response, Nutrient Utilization, and Pathogenesis ofFrontiers in microbiology · 2018Article
- Unique roles of the unfolded protein response pathway in fungal development and differentiation.Scientific reports · 2016Article
- Deubiquitinase Ubp5 Is Required for the Growth and Pathogenicity of Cryptococcus gattii.PloS one · 2016Article
- The endosomal sorting complex required for transport machinery influences haem uptake and capsule elaboration in Cryptococcus neoformans.Molecular microbiology · 2015Article
- Deubiquitinating enzymes as promising drug targets for infectious diseases.Current pharmaceutical design · 2013Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Ubiquitination is a reversible protein modification that influences various cellular processes in eukaryotic cells. Deubiquitinating enzymes remove ubiquitin, maintain ubiquitin homeostasis and regulate protein degradation via the ubiquitination pathway. Cryptococcus neoformans is an important basidiomycete pathogen that causes life-threatening meningoencephalitis primarily in the immunocompromised population. In order to understand the possible influence deubiquitinases have on growth and virulence of the model pathogenic yeast Cryptococcus neoformans, we generated deletion mutants of seven putative deubiquitinase genes. Compared to other deubiquitinating enzyme mutants, a ubp5Δ mutant exhibited severely attenuated virulence and many distinct phenotypes, including decreased capsule formation, hypomelanization, defective sporulation, and elevated sensitivity to several external stressors (such as high temperature, oxidative and nitrosative stresses, high salts, and antifungal agents). Ubp5 is likely the major deubiquitinating enzyme for stress responses in C. neoformans, which further delineates the evolutionary divergence of Cryptococcus from the model yeast S. cerevisiae, and provides an important paradigm for understanding the potential role of deubiquitination in virulence by other pathogenic fungi. Other putative deubiquitinase mutants (doa4Δ and ubp13Δ) share some phenotypes with the ubp5Δ mutant, illustrating functional overlap among deubiquitinating enzymes in C. neoformans. Therefore, deubiquitinating enzymes (especially Ubp5) are essential for the virulence composite of C. neoformans and provide an additional yeast survival and propagation advantage in the host.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.