ArticleNucleic acids research2012
Structural flexibility of RNA as molecular basis for Hfq chaperone function.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Specific and Global RNA Regulators inInternational journal of molecular sciences · 2021Review
- Hfq-dependent mRNA unfolding promotes sRNA-based inhibition of translation.The EMBO journal · 2019Article
- Proteins That Chaperone RNA Regulation.Microbiology spectrum · 2018Review
- The important conformational plasticity of DsrA sRNA for adapting multiple target regulation.Nucleic acids research · 2017Article
- Intermolecular base stacking mediates RNA-RNA interaction in a crystal structure of the RNA chaperone Hfq.Scientific reports · 2017Article
- Article
- Functional and Structural Analysis of a Highly-Expressed Yersinia pestis Small RNA following Infection of Cultured Macrophages.PloS one · 2016Article
- Hfqs in Bacillus anthracis: Role of protein sequence variation in the structure and function of proteins in the Hfq family.Protein science : a publication of the Protein Society · 2015Article
- The Heptameric SmAP1 and SmAP2 Proteins of the Crenarchaeon Sulfolobus Solfataricus Bind to Common and Distinct RNA Targets.Life (Basel, Switzerland) · 2015Article
- Small-angle X-ray scattering: a bridge between RNA secondary structures and three-dimensional topological structures.Current opinion in structural biology · 2015Review
- Regulation of Hfq by the RNA CrcZ in Pseudomonas aeruginosa carbon catabolite repression.PLoS genetics · 2014Article
- Mapping Hfq-RNA interaction surfaces using tryptophan fluorescence quenching.Nucleic acids research · 2014Article
- Duplex formation between the sRNA DsrA and rpoS mRNA is not sufficient for efficient RpoS synthesis at low temperature.RNA biology · 2013Article
- Hfq binding changes the structure ofRNA (New York, N.Y.) · 2013Article
- Conserved arginines on the rim of Hfq catalyze base pair formation and exchange.Nucleic acids research · 2013Article
- Hfq-bridged ternary complex is important for translation activation of rpoS by DsrA.Nucleic acids research · 2013Article
- The nonstructural protein 2C of a Picorna-like virus displays nucleic acid helix destabilizing activity that can be functionally separated from its ATPase activity.Journal of virology · 2013Article
- Cycling of RNAs on Hfq.RNA biology · 2013Review
- Structural mechanism of Staphylococcus aureus Hfq binding to an RNA A-tract.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In enteric bacteria, many small regulatory RNAs (sRNAs) associate with the RNA chaperone host factor Q (Hfq) and often require the protein for regulation of target mRNAs. Previous studies suggested that the hexameric Escherichia coli Hfq (Hfq(Ec)) binds sRNAs on the proximal site, whereas the distal site has been implicated in Hfq-mRNA interactions. Employing a combination of small angle X-ray scattering, nuclear magnetic resonance and biochemical approaches, we report the structural analysis of a 1:1 complex of Hfq(Ec) with a 34-nt-long subsequence of a natural substrate sRNA, DsrA (DsrA(34)). This sRNA is involved in post-transcriptional regulation of the E. coli rpoS mRNA encoding the stationary phase sigma factor RpoS. The molecular envelopes of Hfq(Ec) in complex with DsrA(34) revealed an overall asymmetric shape of the complex in solution with the protein maintaining its doughnut-like structure, whereas the extended DsrA(34) is flexible and displays an ensemble of different spatial arrangements. These results are discussed in terms of a model, wherein the structural flexibility of RNA ligands bound to Hfq stochastically facilitates base pairing and provides the foundation for the RNA chaperone function inherent to Hfq.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.