ArticleNucleic acids research2012
Customized optimization of metabolic pathways by combinatorial transcriptional engineering.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05940701 (The Effectiveness of Biomimetic Materials in Preventing Enamel Demineralization During Fixed Appliance Orthodontic Treatment), which is not on this map. Cited by 95 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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The Effectiveness of Biomimetic Materials in Preventing Enamel Demineralization During Fixed Appliance Orthodontic Treatment: a Randomized Clinical Trial
Who cites it
95 citing papers in PubMed.
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- High yield strategies for triterpenoid biosynthesis in cell factories.Journal of microbiology (Seoul, Korea) · 2026Review
- Exploring chromosomal position effects for predictable tuning of metabolic pathways in yeast.Nucleic acids research · 2026Article
- Deep Generative Model-Driven Design of Microbial Synthetic Promoters.Journal of microbiology and biotechnology · 2025Review
- In vivo pathway optimization in yeast via loxpsym-mediated shuffling of upstream activating sequences.Nucleic acids research · 2025Article
- Effect of biomimetic tooth mousse and toothpaste on the incidence of white spot lesions during fixed appliance orthodontic treatment: A randomized clinical trial.Journal of Taibah University Medical Sciences · 2025Article
- Sub-genomic RNAi-assisted strain evolution of filamentous fungi for enhanced protein production.Applied and environmental microbiology · 2024Article
- Systematic sequence engineering enhances the induction strength of the glucose-regulated GTH1 promoter of Komagataella phaffii.Nucleic acids research · 2023Article
- Design of synthetic promoters for cyanobacteria with generative deep-learning model.Nucleic acids research · 2023Article
- Design of Four Small-Molecule-Inducible Systems in the Yeast Chromosome, Applied to Optimize Terpene Biosynthesis.ACS synthetic biology · 2023Article
- Natural promoters and promoter engineering strategies for metabolic regulation in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2023Review
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- Simultaneous manipulation of multiple genes within a same regulatory stage for iterative evolution of Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2022Article
- Synthetic Biology Tool Development Advances Predictable Gene Expression in the Metabolically Versatile Soil BacteriumFrontiers in bioengineering and biotechnology · 2022Article
- Biosynthesis of a novel ganoderic acid by expressing CYP genes from Ganoderma lucidum in Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2022Article
- Review
- Multiple Effects of Ascorbic Acid against Chronic Diseases: Updated Evidence from Preclinical and Clinical Studies.Antioxidants (Basel, Switzerland) · 2020Review
- Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Synthetic promoter design in Escherichia coli based on a deep generative network.Nucleic acids research · 2020Article
35 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major challenge in metabolic engineering and synthetic biology is to balance the flux of an engineered heterologous metabolic pathway to achieve high yield and productivity in a target organism. Here, we report a simple, efficient and programmable approach named 'customized optimization of metabolic pathways by combinatorial transcriptional engineering (COMPACTER)' for rapid tuning of gene expression in a heterologous pathway under distinct metabolic backgrounds. Specifically, a library of mutant pathways is created by de novo assembly of promoter mutants of varying strengths for each pathway gene in a target organism followed by high-throughput screening/selection. To demonstrate this approach, a single round of COMPACTER was used to generate both a xylose utilizing pathway with near-highest efficiency and a cellobiose utilizing pathway with highest efficiency that were ever reported in literature for both laboratory and industrial yeast strains. Interestingly, these engineered xylose and cellobiose utilizing pathways were all host-specific. Therefore, COMPACTER represents a powerful approach to tailor-make metabolic pathways for different strain backgrounds, which is difficult if not impossible to achieve by existing pathway engineering methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.