ArticleJournal of clinical microbiology2012
Detection of extended-spectrum β-lactamase and Klebsiella pneumoniae Carbapenemase genes directly from blood cultures by use of a nucleic acid microarray.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Emerging strategies for the noninvasive diagnosis of nosocomial pneumonia.Expert review of anti-infective therapy · 2019Pooled it
- Rapid commercial CTX-M diagnostics: Performance, limitations and clinical impact.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Review
- Recent Advances in the Use of Molecular Methods for the Diagnosis of Bacterial Infections.Pathogens (Basel, Switzerland) · 2022Review
- Molecules that Inhibit Bacterial Resistance Enzymes.Molecules (Basel, Switzerland) · 2018Review
- New Molecular Diagnostic Approaches to Bacterial Infections and Antibacterial Resistance.Annual review of medicine · 2018Review
- Direct detection of extended-spectrum beta-lactamases (CTX-M) from blood cultures by LC-MS/MS bottom-up proteomics.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- Modeling risk for developing drug resistant bacterial infections in an MDR-naive critically ill population.Therapeutic advances in infectious disease · 2017Article
- Intestinal Carriage of Carbapenemase-Producing Organisms: Current Status of Surveillance Methods.Clinical microbiology reviews · 2016Review
- Klebsiella pneumoniae bloodstream infection: epidemiology and impact of inappropriate empirical therapy.Medicine · 2014Review
- Non-phenotypic tests to detect and characterize antibiotic resistance mechanisms in Enterobacteriaceae.Diagnostic microbiology and infectious disease · 2013Review
- Saponin promotes rapid identification and antimicrobial susceptibility profiling of Gram-positive and Gram-negative bacteria in blood cultures with the Vitek 2 system.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Article
- Evaluation of a microarray-based assay for rapid identification of Gram-positive organisms and resistance markers in positive blood cultures.Journal of clinical microbiology · 2013Article
- Rapid and simultaneous detection of genes encoding Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) in Gram-negative bacilli.Journal of clinical microbiology · 2013Article
- Review
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5 authors.
Funding
Abstract
The growing crisis of multidrug-resistant (MDR) Gram-negative bacteria requires that current technologies permit the rapid detection of extended-spectrum β-lactamase (bla(ESBL)) and Klebsiella pneumoniae carbapenemase (bla(KPC)) genes. In the present study, we assessed the performance characteristics of a commercially available nucleic acid microarray system for the detection of bla(ESBL) and bla(KPC) genes directly from positive blood cultures. Using blood cultures (BCs) that contained Gram-negative bacilli identified by Gram staining, we isolated bacterial DNA using spin columns (BC-C) and rapid water lysis (BC-W). Twenty ESBL/KPC-positive and 20 ESBL/KPC-negative blood culture samples, as well as 20 non-lactose-fermenting organisms, were tested. The 20 isolates that were ESBL positive by phenotypic testing were also evaluated on solid medium (SM), and the DNA was extracted by use of a spin column (SM-C). The resulting 140 DNA extractions were assessed for DNA quantity and quality using 260/280-nm absorbance ratios, and DNA microarray analysis was performed in a blinded fashion. Microarray and phenotypic results were concordant for 98.3% of BC-W, 90% of BC-C, and 95% of SM-C samples. Compared to phenotypic testing, the sensitivity and specificity for BC-C samples were 88.9% and 100%, respectively, and for BC-W samples, the sensitivity and specificity were 94.4% and 100%, respectively. BC-W samples yielded the highest concordance with phenotypic results. Nucleic acid microarrays offer promise in the identification of bla(ESBL) and bla(KPC) genes directly from blood cultures, thereby reducing the time to identification of these important pathogens.
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