ArticleJournal of clinical microbiology2012
Optimization of the preanalytical steps of matrix-assisted laser desorption ionization-time of flight mass spectrometry identification provides a flexible and efficient tool for identification of clinical yeast isolates in medical laboratories.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Silent Outbreaks ofAntibiotics (Basel, Switzerland) · 2026Article
- Mass Spectrometry: A Rosetta Stone to Learn How Fungi Interact and Talk.Life (Basel, Switzerland) · 2020Review
- Pushing the Limits of MALDI-TOF Mass Spectrometry: Beyond Fungal Species Identification.Journal of fungi (Basel, Switzerland) · 2015Review
- Elucidating the Ramularia eucalypti species complex.Persoonia · 2015Article
- Insufficient Discriminatory Power of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Dendrograms to Determine the Clonality of Multi-Drug-Resistant Acinetobacter baumannii Isolates from an Intensive Care Unit.BioMed research international · 2015Article
- Yeast on-target lysis (YOTL), a procedure for making auxiliary mass spectrum data sets for clinical routine identification of yeasts.Journal of clinical microbiology · 2014Article
- Development and validation of an in-house database for matrix-assisted laser desorption ionization-time of flight mass spectrometry-based yeast identification using a fast protein extraction procedure.Journal of clinical microbiology · 2014Article
- Article
- Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology.Clinical microbiology reviews · 2013Review
- Comparative evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry systems for identification of yeasts of medical importance.Journal of clinical microbiology · 2013Article
- Multicenter study evaluating the Vitek MS system for identification of medically important yeasts.Journal of clinical microbiology · 2013Article
- Advances in identification of clinical yeast isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.Journal of clinical microbiology · 2013Article
- Comparison between the Biflex III-Biotyper and the Axima-SARAMIS systems for yeast identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry.Journal of clinical microbiology · 2013Article
- Microbiota of table olive fermentations and criteria of selection for their use as starters.Frontiers in microbiology · 2013Article
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6 authors.
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Abstract
We report here that modifications of the preanalytical steps of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification of yeasts, with regard to the original protocol provided by the manufacturers, appear to be efficient for the reliable routine identification of clinical yeast isolates in medical laboratories. Indeed, when one colony was sampled instead of five and the protein extraction protocol was modified, the performance of MALDI-TOF MS was superior to that of the API ID 32C method (discrepancies were confirmed by using molecular identification), allowing the correct identification of 94% of the 335 clinical isolates prospectively tested. We then demonstrated that the time for which the primary cultures were preincubated on CHROMagar did not impact the identification of yeasts by MALDI-TOF MS, since 95.1 and 96.2% of the 183 clinical yeast isolates prospectively tested were correctly identified after 48 and 72 h of preincubation, respectively.
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