ArticleThe Journal of biological chemistry2012
The fluorescein-derived dye aminophenyl fluorescein is a suitable tool to detect hypobromous acid (HOBr)-producing activity in eosinophils.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- β2 integrin-mediated cell-cell contact transfers active myeloperoxidase from neutrophils to endothelial cells.The Journal of biological chemistry · 2013Trial
- Human serum albumin modified in myeloperoxidase-dependent reactions is a mediator of neutrophil extracellular trap formation.Journal of biomedical research · 2026Article
- Mechanism of peroxidasin inactivation in hyperglycemia: Heme damage by reactive oxygen species.Biochemical and biophysical research communications · 2023Article
- Identification of brominated proteins in renal extracellular matrix: Potential interactions with peroxidasin.Biochemical and biophysical research communications · 2023Article
- New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent "Turn-On" Probe for Endogenous Detection of HOCl and Reactive Halogenated Species.Antioxidants (Basel, Switzerland) · 2022Article
- Measuring peroxidasin activity in live cells using bromide addition for signal amplification.Redox biology · 2022Article
- Fluorescent probes for monitoring myeloperoxidase-derived hypochlorous acid: a comparative study.Scientific reports · 2022Article
- An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation.ChemCatChem · 2020Article
- Top-Down Characterization of an Antimicrobial Sanitizer, Leading From Quenchers of Efficacy to Mode of Action.Frontiers in microbiology · 2020Article
- State-of-the-art: functional fluorescent probes for bioimaging and pharmacological research.Acta pharmacologica Sinica · 2019Review
- Small-molecule luminescent probes for the detection of cellular oxidizing and nitrating species.Free radical biology & medicine · 2018Review
- Inhibitory Anti-Peroxidasin Antibodies in Pulmonary-Renal Syndromes.Journal of the American Society of Nephrology : JASN · 2018Article
- Oxygen atoms are critical in rendering THP-1 leukaemia cells susceptible to cold physical plasma-induced apoptosis.Scientific reports · 2017Article
- Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples.Journal of visualized experiments : JoVE · 2016Article
- Methods for measuring myeloperoxidase activity toward assessing inhibitor efficacy in living systems.Journal of leukocyte biology · 2016Review
- Myeloperoxidase scavenges peroxynitrite: A novel anti-inflammatory action of the heme enzyme.Archives of biochemistry and biophysics · 2015Article
- Mechanism of reaction of chlorite with mammalian heme peroxidases.Journal of inorganic biochemistry · 2014Article
- Transiently produced hypochlorite is responsible for the irreversible inhibition of chlorite dismutase.Biochemistry · 2014Article
- S-nitrosylation regulates mitochondrial quality control via activation of parkin.Scientific reports · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The specific detection of peroxidase activity in human granulocytes is essential to elucidate their role in innate immune responses, immune regulation, and inflammatory diseases. The halogenating activity of myeloperoxidase in neutrophils can be determined by the novel fluorescent probe aminophenyl fluorescein (APF). Thereby non-fluorescent APF is oxidized by HOCl to form fluorescein. We successfully verified that APF equally detects the hypobromous acid (HOBr)-producing activity of eosinophil granulocytes. This was revealed by three different approaches. First, we investigated the conversion of non-fluorescent APF into fluorescein by HOCl and HOBr by means of fluorescence and mass spectrometry approaches. Thereby comparable chemical mechanisms were observed for both acids. Furthermore in vitro kinetic studies were used to detect the halogenating activity of myeloperoxidase and eosinophil peroxidase by using APF. Here the dye well reflected the different substrate specificities of myeloperoxidase and eosinophil peroxidase regarding chloride and bromide. Finally, peroxidase activities were successfully detected in phorbol ester-stimulated neutrophils and eosinophils using flow cytometry. Thereby inhibitory studies confirmed the peroxidase-dependent oxidation of APF. To sum up, APF is a promising tool for further evaluation of the halogenating activity of peroxidases in both neutrophils and eosinophils.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.