ArticleMolecular microbiology2012
Evidence for roles of the Escherichia coli Hda protein beyond regulatory inactivation of DnaA.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 16 citations in OpenAlex.
- The loop domains of the E. coli β sliding clamp are required for Hda-dependent inactivation of DnaA.Genetics · 2026Article
- Negative DNA supercoiling enhances DARS2 binding of DNA-bending protein IHF in the activation of Fis-dependent ATP-DnaA production.Nucleic acids research · 2025Article
- IHF and Fis asInternational journal of molecular sciences · 2023Review
- The Mutant βJournal of bacteriology · 2021Article
- Elevated Levels of the Escherichia coliJournal of bacteriology · 2021Article
- Maladaptive DNA repair is the ultimate contributor to the death of trimethoprim-treated cells under aerobic and anaerobic conditions.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Insufficient levels of the nrdAB-encoded ribonucleotide reductase underlie the severe growth defect of the Δhda E. coli strain.Molecular microbiology · 2017Article
- Dynamic assembly of Hda and the sliding clamp in the regulation of replication licensing.Nucleic acids research · 2017Article
- The DnaA Cycle inFrontiers in microbiology · 2017Review
- The Caulobacter crescentus Homolog of DnaA (HdaA) Also Regulates the Proteolysis of the Replication Initiator Protein DnaA.Journal of bacteriology · 2015Article
- A Genetic Selection for dinB Mutants Reveals an Interaction between DNA Polymerase IV and the Replicative Polymerase That Is Required for Translesion Synthesis.PLoS genetics · 2015Article
- Oxidative DNA damage is instrumental in hyperreplication stress-induced inviability of Escherichia coli.Nucleic acids research · 2014Article
- The β-sliding clamp directs the localization of HdaA to the replisome in Caulobacter crescentus.Microbiology (Reading, England) · 2013Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The ATP-bound form of the Escherichia coli DnaA protein binds 'DnaA boxes' present in the origin of replication (oriC) and operator sites of several genes, including dnaA, to co-ordinate their transcription with initiation of replication. The Hda protein, together with the β sliding clamp, stimulates the ATPase activity of DnaA via a process termed regulatory inactivation of DnaA (RIDA), to regulate the activity of DnaA in DNA replication. Here, we used the mutant dnaN159 strain, which expresses the β159 clamp protein, to gain insight into how the actions of Hda are co-ordinated with replication. Elevated expression of Hda impeded growth of the dnaN159 strain in a Pol II- and Pol IV-dependent manner, suggesting a role for Hda managing the actions of these Pols. In a wild-type strain, elevated levels of Hda conferred sensitivity to nitrofurazone, and suppressed the frequency of -1 frameshift mutations characteristic of Pol IV, while loss of hda conferred cold sensitivity. Using the dnaN159 strain, we identified 24 novel hda alleles, four of which supported E. coli viability despite their RIDA defect. Taken together, these findings suggest that although one or more Hda functions are essential for cell viability, RIDA may be dispensable.
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