ArticleMolecular brain2012
Rapid and efficient genetic manipulation of gyrencephalic carnivores using in utero electroporation.
Article in Molecular brain, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 91 citations in OpenAlex.
- Ferrets and genetically modified ferrets as model organisms in biomedical research: a review.Frontiers in genome editing · 2026Review
- Species-Specific Parent-Of-Origin Expression of Adam23 in the Mammalian Brain.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- Investigation of the mechanisms underlying the development and evolution of the mammalian cerebrum using gyrencephalic ferrets.Frontiers in cell and developmental biology · 2026Review
- Shaping the Neocortex: Radial Glia and Astrocytes in Development and Evolution.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Review
- Evolutionary changes leading to efficient glymphatic circulation in the mammalian brain.Nature communications · 2024Article
- Conserved transcriptional regulation by BRN1 and BRN2 in neocortical progenitors drives mammalian neural specification and neocortical expansion.Nature communications · 2024Article
- Truncated radial glia as a common precursor in the late corticogenesis of gyrencephalic mammals.eLife · 2023Article
- Advanced Techniques Using In Vivo Electroporation to Study the Molecular Mechanisms of Cerebral Development Disorders.International journal of molecular sciences · 2023Review
- Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification.Journal of visualized experiments : JoVE · 2023Article
- Orchestrated neuronal migration and cortical folding: A computational and experimental study.PLoS computational biology · 2022Article
- Localized astrogenesis regulates gyrification of the cerebral cortex.Science advances · 2022Article
- Orchestration of Ion Channels and Transporters in Neocortical Development and Neurological Disorders.Frontiers in neuroscience · 2022Review
- Isolation of ferret astrocytes reveals their morphological, transcriptional, and functional differences from mouse astrocytes.Frontiers in cellular neuroscience · 2022Article
- Investigation of the Mechanisms Underlying the Development and Evolution of the Cerebral Cortex Using Gyrencephalic Ferrets.Frontiers in cell and developmental biology · 2022Review
- A protocol forSTAR protocols · 2021Article
- The expression of aristaless-related homeobox in neural progenitors of gyrencephalic carnivore ferrets.Biochemistry and biophysics reports · 2021Article
- Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation.Scientific reports · 2021Article
- The Ferret as a Model System for Neocortex Development and Evolution.Frontiers in cell and developmental biology · 2021Review
- Roots of the Malformations of Cortical Development in the Cell Biology of Neural Progenitor Cells.Frontiers in neuroscience · 2021Review
- A discrete subtype of neural progenitor crucial for cortical folding in the gyrencephalic mammalian brain.eLife · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHigher mammals such as primates and carnivores have highly developed unique brain structures such as the ocular dominance columns in the visual cortex, and the gyrus and outer subventricular zone of the cerebral cortex. However, our molecular understanding of the formation, function and diseases of these structures is still limited, mainly because genetic manipulations that can be applied to higher mammals are still poorly available.
resultsHere we developed and validated a rapid and efficient technique that enables genetic manipulations in the brain of gyrencephalic carnivores using in utero electroporation. Transgene-expressing ferret babies were obtained within a few weeks after electroporation. GFP expression was detectable in the embryo and was observed at least 2 months after birth. Our technique was useful for expressing transgenes in both superficial and deep cortical neurons, and for examining the dendritic morphologies and axonal trajectories of GFP-expressing neurons in ferrets. Furthermore, multiple genes were efficiently co-expressed in the same neurons.
conclusionOur method promises to be a powerful tool for investigating the fundamental mechanisms underlying the development, function and pathophysiology of brain structures which are unique to higher mammals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.