ArticleMolecular microbiology2012
The ABC transporter MalFGK(2) sequesters the MalT transcription factor at the membrane in the absence of cognate substrate.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 31 citations in OpenAlex.
- The Evolution of ABC Importers.Journal of molecular biology · 2025Review
- Structurally diverse C-terminal accessory domains in type I ABC importers reveal distinct regulatory mechanisms.Structure (London, England : 1993) · 2025Review
- Structural basis for negative regulation of the Escherichia coli maltose system.Nature communications · 2023Article
- Glucose Transport inMicroorganisms · 2023Review
- Transporters of glucose and other carbohydrates in bacteria.Pflugers Archiv : European journal of physiology · 2020Review
- Parallel experimental evolution reveals a novel repressive control of GalP on xylose fermentation in Escherichia coli.Biotechnology and bioengineering · 2019Article
- Double autoinhibition mechanism of signal transduction ATPases with numerous domains (STAND) with a tetratricopeptide repeat sensor.Nucleic acids research · 2019Article
- The α-glucan phosphorylase MalP of Corynebacterium glutamicum is subject to transcriptional regulation and competitive inhibition by ADP-glucose.Journal of bacteriology · 2015Article
- Coordinated transporter activity shapes high-affinity iron acquisition in cyanobacteria.The ISME journal · 2014Article
- Article
- Phosphatidylglycerol directs binding and inhibitory action of EIIAGlc protein on the maltose transporter.The Journal of biological chemistry · 2013Article
- Carbohydrate kinase (RhaK)-dependent ABC transport of rhamnose in Rhizobium leguminosarum demonstrates genetic separation of kinase and transport activities.Journal of bacteriology · 2013Article
- Dynamic localization of a transcription factor in Bacillus subtilis: the LicT antiterminator relocalizes in response to inducer availability.Journal of bacteriology · 2013Article
- Comprehensive mutational analysis of sucrose-metabolizing pathways in Streptococcus mutans reveals novel roles for the sucrose phosphotransferase system permease.Journal of bacteriology · 2013Article
- An integrated transport mechanism of the maltose ABC importer.Research in microbiologyReview
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3 authors at 3 institutions in 2 countries.
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Abstract
MalK, the cytoplasmic component of the maltose ABC transporter from Escherichia coli is known to control negatively the activity of MalT, the activator of the maltose regulon, through complex formation. Here we further investigate this regulatory process by monitoring MalT activity and performing fluorescence microscopy analyses under various conditions. We establish that, under physiological conditions, the molecular entity that interacts with MalT is not free MalK, but the maltose transporter, MalFGK(2) , which sequesters MalT to the membrane. Furthermore, we provide compelling evidence that the transporter's ability to bind MalT is not constitutive, but strongly diminished when MalFGK(2) is engaged in sugar transport. Notably, the outward-facing transporter, i.e. the catalytic intermediate, is ineffective in inhibiting MalT compared to the inward-facing state, i.e. the resting form. Analyses of available genetic and structural data suggest how the interaction between one inactive MalT molecule and MalFGK(2) would be sensitive to the transporter state, thereby allowing MalT release upon maltose entrance. A related mechanism may underpin signalling by other ABC transporters.
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