ArticleThe Plant cell2012
A PP6-type phosphatase holoenzyme directly regulates PIN phosphorylation and auxin efflux in Arabidopsis.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 93 citations in OpenAlex.
- Beyond membrane transport: Transporters as dynamic signal integrators for plant signaling and adaptation.Plant communications · 2026Review
- Reversible phosphorylation of NPH3/RPT2-like proteins regulates phototropin receptor signaling.The Plant cell · 2026Article
- Genomic basis underlying alternative transcripts-mediated drought tolerance in maize.Genome biology · 2026Article
- Hyperoside, a flavonoid, inducesScience advances · 2025Article
- Protein phosphatase PP2C19 controls hypocotyl phototropism through the phosphorylation modification of NONPHOTOTROPIC HYPOCOTYL3 in Arabidopsis.Plant & cell physiology · 2025Article
- PINOID-centered genetic interactions mediate auxin action in cotyledon formation.Plant direct · 2024Review
- Phosphorylation status of Bβ subunit acts as a switch to regulate the function of phosphatase PP2A in ethylene-mediated root growth inhibition.The New phytologist · 2022Article
- Auxin Transporters-A Biochemical View.Cold Spring Harbor perspectives in biology · 2022Review
- Subcellular trafficking and post-translational modification regulate PIN polarity in plants.Frontiers in plant science · 2022Review
- Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs.International journal of molecular sciences · 2021Review
- Article
- One Hundred Candidate Genes and Their Roles in Drought and Salt Tolerance in Wheat.International journal of molecular sciences · 2021Review
- Auxin and Root Gravitropism: Addressing Basic Cellular Processes by Exploiting a Defined Growth Response.International journal of molecular sciences · 2021Review
- Integrated Bioinformatics Analyses ofFrontiers in plant science · 2021Article
- All Roads Lead to Auxin: Post-translational Regulation of Auxin Transport by Multiple Hormonal Pathways.Plant communications · 2020Review
- Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.The Plant cell · 2020Article
- The lipid code-dependent phosphoswitch PDK1-D6PK activates PIN-mediated auxin efflux in Arabidopsis.Nature plants · 2020Article
- Remove, Recycle, Degrade: Regulating Plasma Membrane Protein Accumulation.The Plant cell · 2019Review
- Article
- The Maize Clade A PP2C Phosphatases Play Critical Roles in Multiple Abiotic Stress Responses.International journal of molecular sciences · 2019Article
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Authors and funding
16 authors at 8 institutions in 4 countries.
Funding
Abstract
The directional transport of the phytohormone auxin depends on the phosphorylation status and polar localization of PIN-FORMED (PIN) auxin efflux proteins. While PINIOD (PID) kinase is directly involved in the phosphorylation of PIN proteins, the phosphatase holoenzyme complexes that dephosphorylate PIN proteins remain elusive. Here, we demonstrate that mutations simultaneously disrupting the function of Arabidopsis thaliana FyPP1 (for Phytochrome-associated serine/threonine protein phosphatase1) and FyPP3, two homologous genes encoding the catalytic subunits of protein phosphatase6 (PP6), cause elevated accumulation of phosphorylated PIN proteins, correlating with a basal-to-apical shift in subcellular PIN localization. The changes in PIN polarity result in increased root basipetal auxin transport and severe defects, including shorter roots, fewer lateral roots, defective columella cells, root meristem collapse, abnormal cotyledons (small, cup-shaped, or fused cotyledons), and altered leaf venation. Our molecular, biochemical, and genetic data support the notion that FyPP1/3, SAL (for SAPS DOMAIN-LIKE), and PP2AA proteins (RCN1 [for ROOTS CURL IN NAPHTHYLPHTHALAMIC ACID1] or PP2AA1, PP2AA2, and PP2AA3) physically interact to form a novel PP6-type heterotrimeric holoenzyme complex. We also show that FyPP1/3, SAL, and PP2AA interact with a subset of PIN proteins and that for SAL the strength of the interaction depends on the PIN phosphorylation status. Thus, an Arabidopsis PP6-type phosphatase holoenzyme acts antagonistically with PID to direct auxin transport polarity and plant development by directly regulating PIN phosphorylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.