ArticleGenes & development2012
fus/TLS orchestrates splicing of developmental regulators during gastrulation.
Article in Genes & development, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- A postzygotic de novo NCDN mutation identified in a sporadic FTLD patient results in neurochondrin haploinsufficiency and altered FUS granule dynamics.Acta neuropathologica communications · 2022Article
- An autoregulation loop in fust-1 for circular RNA regulation in Caenorhabditis elegans.Genetics · 2021Article
- The atypical RNA-binding protein Taf15 regulates dorsoanterior neural development through diverse mechanisms in Xenopus tropicalis.Development (Cambridge, England) · 2021Article
- FUS regulates autophagy by mediating the transcription of genes critical to the autophagosome formation.Journal of neurochemistry · 2021Article
- The multifaceted role of PARP1 in RNA biogenesis.Wiley interdisciplinary reviews. RNA · 2021Review
- Lysine acetylation regulates the RNA binding, subcellular localization and inclusion formation of FUS.Human molecular genetics · 2020Article
- The ABCF gene family facilitates disaggregation during animal development.Molecular biology of the cell · 2020Review
- Cellular consequences of arginine methylation.Cellular and molecular life sciences : CMLS · 2019Review
- Exploring the suitability of RanBP2-type Zinc Fingers for RNA-binding protein design.Scientific reports · 2019Article
- FUS Regulates Activity of MicroRNA-Mediated Gene Silencing.Molecular cell · 2018Article
- Article
- Clinical and experimental studies of a novel P525R FUS mutation in amyotrophic lateral sclerosis.Neurology. Genetics · 2017Article
- Proteomic analysis of FUS interacting proteins provides insights into FUS function and its role in ALS.Biochimica et biophysica acta · 2016Article
- Mechanisms of FUS mutations in familial amyotrophic lateral sclerosis.Brain research · 2016Review
- Circular RNA Expression: Its Potential Regulation and Function.Trends in genetics : TIG · 2016Review
- The alternative splicing regulator Tra2b is required for somitogenesis and regulates splicing of an inhibitory Wnt11b isoform.Cell reports · 2015Article
- The splicing factor PQBP1 regulates mesodermal and neural development through FGF signaling.Development (Cambridge, England) · 2014Article
- RNA-binding proteins in pluripotency, differentiation, and reprogramming.Frontiers in biology · 2014Article
- Spalt-like 4 promotes posterior neural fates via repression of pou5f3 family members in Xenopus.Development (Cambridge, England) · 2014Article
- ALS-associated mutation FUS-R521C causes DNA damage and RNA splicing defects.The Journal of clinical investigation · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Here we investigated the function of the atypical RNA-binding protein fus/TLS (fused in sarcoma/translocated in sarcoma) during early frog development. We found that fus is necessary for proper mRNA splicing of a set of developmental regulatory genes during early frog development and gastrulation. Upon fus knockdown, embryos fail to gastrulate and show mesodermal differentiation defects that we connect to intron retention in fgf8 (fibroblast growth factor 8) and fgfr2 (fgf receptor 2) transcripts. During gastrulation, the animal and marginal regions dissociate, and we show that this is caused, at least in part, by intron retention in cdh1 transcripts. We confirm the specificity of splicing defects at a genomic level using analysis of RNA sequencing (RNA-seq) and show that 3%-5% of all transcripts display intron retention throughout the pre-mRNA. By analyzing gene ontology slim annotations, we show that the affected genes are enriched for developmental regulators and therefore represent a biologically coherent set of targets for fus regulation in embryogenesis. This shows that fus is central to embryogenesis and may provide information on its function in neurodegenerative disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.