ArticleThe EMBO journal2012
Dephosphorylation of Cdc20 is required for its C-box-dependent activation of the APC/C.
Article in The EMBO journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 120 citations in OpenAlex.
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- A murine cytomegalovirus cell cycle regulator (m54.5p) evolved within the conserved viral DNA polymerase gene.PLoS pathogens · 2026Article
- No transcription, no problem: Protein phosphorylation changes and the transition from oocyte to embryo.Current topics in developmental biology · 2025Review
- Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses.The Journal of cell biology · 2024Article
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- Spatial control of the APC/C ensures the rapid degradation of cyclin B1.The EMBO journal · 2024Article
- The Mitotic Checkpoint Complex controls the association of Cdc20 regulatory protein with the ubiquitin ligase APC/C in mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Analysis of nondegradable cyclins reveals distinct roles of the mitotic cyclins in Drosophila meiosis.G3 (Bethesda, Md.) · 2024Article
- MAPK-dependent control of mitotic progression in S. pombe.BMC biology · 2024Article
- Spo13/MEIKIN ensures a Two-Division meiosis by preventing the activation of APC/CThe EMBO journal · 2023Article
- BUB-1-bound PLK-1 directs CDC-20 kinetochore recruitment to ensure timely embryonic mitoses.Current biology : CB · 2023Article
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- RNA localization to the mitotic spindle is essential for early development and is regulated by kinesin-1 and dynein.Journal of cell science · 2023Article
- Cystathionine β-synthase is required for oocyte quality by ensuring proper meiotic spindle assembly.Cell proliferation · 2022Article
- Role of phosphorylation of Cdc20 in the regulation of the action of APC/C in mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Structural, enzymatic and spatiotemporal regulation of PP2A-B55 phosphatase in the control of mitosis.Frontiers in cell and developmental biology · 2022Review
- Dynamic regulation of mitotic ubiquitin ligase APC/C by coordinated Plx1 kinase and PP2A phosphatase action on a flexible Apc1 loop.The EMBO journal · 2021Article
- Coupling of Cdc20 inhibition and activation by BubR1.The Journal of cell biology · 2021Article
- Tank Binding Kinase 1 modulates spindle assembly checkpoint components to regulate mitosis in breast and lung cancer cells.Biochimica et biophysica acta. Molecular cell research · 2021Article
- Bub1 kinase in the regulation of mitosis.Animal cells and systems · 2021Review
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase is tightly regulated to ensure programmed proteolysis in cells. The activity of the APC/C is positively controlled by cyclin-dependent kinase (CDK), but a second level of control must also exist because phosphorylation inactivates Cdc20, a mitotic APC/C co-activator. How Cdc20 is dephosphorylated specifically, when CDK is high, has remained unexplained. Here, we show that phosphatases are crucial to activate the APC/C. Cdc20 is phosphorylated at six conserved residues (S50/T64/T68/T79/S114/S165) by CDK in Xenopus egg extracts. When all the threonine residues are phosphorylated, Cdc20 binding to and activation of the APC/C are inhibited. Their dephosphorylation is regulated depending on the sites and protein phosphatase 2A, active in mitosis, is essential to dephosphorylate the threonine residues and activate the APC/C. Consistently, most of the Cdc20 bound to the APC/C in anaphase evades phosphorylation at T79. Furthermore, we show that the 'activation domain' of Cdc20 associates with the Apc6 and Apc8 core subunits. Our data suggest that dephosphorylation of Cdc20 is required for its loading and activation of the APC/C ubiquitin ligase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.