ArticleNature communications2012
Visualizing specific protein glycoforms by transmembrane fluorescence resonance energy transfer.
Article in Nature communications, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 115 citations in OpenAlex.
- Investigation Into the Dynamics of the Cupula in the Vestibular Organ of Adult Zebrafish Using Metabolic Glycoengineering.Angewandte Chemie (International ed. in English) · 2026Article
- In-situ profiling of glycosylation on single cells with surface plasmon resonance imaging.Nature communications · 2025Article
- Glycosylation: mechanisms, biological functions and clinical implications.Signal transduction and targeted therapy · 2024Review
- In Situ Glycan Analysis and Editing in Living Systems.JACS Au · 2024Review
- Engineering of cell-surface receptors for analysis of receptor internalization and detection of receptor-specific glycosylation.Chemical science · 2024Article
- Visualization of multiple localizations of GLUT4 by fluorescent probes of PYP-tag with designed unnatural warhead.Chemical science · 2023Article
- Molecular probes for cellular imaging of post-translational proteoforms.RSC chemical biology · 2022Review
- Heparin impairs skeletal muscle glucose uptake by inhibiting insulin binding to insulin receptor.Endocrinology, diabetes & metabolism · 2021Article
- Exploring the Potential of β-CateninMolecules (Basel, Switzerland) · 2020Article
- Fluorometric visualization of mucin 1 glycans on cell surfaces based on rolling-mediated cascade amplification and CdTe quantum dots.Mikrochimica acta · 2019Article
- Article
- Tools for Studying Glycans: Recent Advances in Chemoenzymatic Glycan Labeling.ACS chemical biology · 2017Review
- Imaging specific newly synthesized proteins within cells by fluorescence resonance energy transfer.Chemical science · 2017Article
- Exploring Drug-Receptor Interaction Kinetics: Lessons from a Sigma-1 Receptor Transmembrane Biosensor.Frontiers in pharmacology · 2017Article
- Chemical Glycoproteomics.Chemical reviews · 2016Review
- Spatiotemporal control of cell-cell reversible interactions using molecular engineering.Nature communications · 2016Article
- Fluorogenic probes reveal a role of GLUT4 N-glycosylation in intracellular trafficking.Nature chemical biology · 2016Article
- Designing sugar mimetics: non-natural pyranosides as innovative chemical tools.Current opinion in chemical biology · 2016Review
- Single-molecule visualization of dynamic transitions of pore-forming peptides among multiple transmembrane positions.Nature communications · 2016Article
- Applying DNA rolling circle amplification in fluorescence imaging of cell surface glycans labeled by a metabolic method.Chemical science · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analyses of mice lacking glycosyltransferase have suggested that their pathological phenotypes are not attributable to the overall change of the sugar modification, but instead the result of changes of the glycan structures on a specific 'target' glycoprotein. Therefore, detecting or monitoring the glycosylation status of a specific protein in living cells is important, but no such methods are currently available. Here we demonstrate the detection of glycoforms of a specific glycoprotein using the fluorescence resonance energy transfer technique. Using model proteins, we detect characteristic fluorescence resonance energy transfer signals from the specific glycoform-bearing target glycoprotein. We also show that, upon insulin removal, sialylated glycoforms of green fluorescent protein-tagged GLUT4 seem to be internalized more slowly than non-sialylated GLUT4. This novel analytical imaging tool allows studying the roles of specific glycan modifications of a protein of interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.