ArticleBiophysical journal2012
Oxygen depletion triggers switching between discrete speed modes of gonococcal type IV pili.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
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- Anti-Virulence Strategy of Novel Dehydroabietic Acid Derivatives: Design, Synthesis, and Antibacterial Evaluation.International journal of molecular sciences · 2023Article
- External Stresses Affect Gonococcal Type 4 Pilus Dynamics.Frontiers in microbiology · 2022Article
- Modelling bacterial twitching in fluid flows: a CFD-DEM approach.Scientific reports · 2019Article
- Motor Properties of PilT-Independent Type 4 Pilus Retraction in Gonococci.Journal of bacteriology · 2019Article
- Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease.Nature microbiology · 2019Article
- Assessing Travel Conditions: Environmental and Host Influences On Bacterial Surface Motility.Journal of bacteriology · 2018Article
- Role of Cyclic Di-GMP and Exopolysaccharide in Type IV Pilus Dynamics.Journal of bacteriology · 2017Article
- Emergence of complex behavior in pili-based motility in early stages of P. aeruginosa surface adaptation.Scientific reports · 2017Article
- Article
- Kinetics of DNA uptake during transformation provide evidence for a translocation ratchet mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Cyclic diguanylate signaling in Gram-positive bacteria.FEMS microbiology reviews · 2016Review
- Gene Transfer Efficiency in Gonococcal Biofilms: Role of Biofilm Age, Architecture, and Pilin Antigenic Variation.Journal of bacteriology · 2015Article
- Characterization of motility and piliation in pathogenic Neisseria.BMC microbiology · 2015Article
- Conserved Streptococcus pneumoniae spirosomes suggest a single type of transformation pilus in competence.PLoS pathogens · 2015Article
- Uptake of extracellular DNA: competence induced pili in natural transformation of Streptococcus pneumoniae.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- Article
- Secretion of a pneumococcal type II secretion system pilus correlates with DNA uptake during transformation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type IV pili are polymeric bacterial appendages that affect host cell interaction, motility, biofilm formation, and horizontal gene transfer. These force-generating motors work in at least three distinct velocity modes-elongation, and retraction at two distinct speeds, high and low. Yet it is unclear which regulatory inputs control their speeds. Here, we addressed this question for the human pathogen Neisseria gonorrhoeae. Using a combination of image analysis and surface analytics, we simultaneously monitored the speed of twitching motility and the concentration of oxygen. While oxygen was detectable, bacteria moved in the high-speed mode (1.5 μm/s). Upon full depletion of oxygen, gonococci simultaneously switched into the low-speed mode (0.5 μm/s). Speed switching was complete within seconds, independent of transcription, and reversible upon oxygen restoration. Using laser tweezers, we found that oxygen depletion triggered speed switching of the pilus motor at the single-molecule level. In the transition regime, single pili switched between both modes, indicating bistability. Switching is well described by a two-state model whereby the oxygen level controls the occupancy of the states.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.