ArticleBioscience reports2012
Characterization of secretory sphingomyelinase activity, lipoprotein sphingolipid content and LDL aggregation in ldlr-/- mice fed on a high-fat diet.
Article in Bioscience reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
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- The Lard Works in Mysterious Ways: Ceramides in Nutrition-Linked Chronic Disease.Annual review of nutrition · 2022Review
- Metabolomic and proteomic characterization of sng and pain phenotypes in fibromyalgia.European journal of pain (London, England) · 2022Article
- Preparation of LDL , Oxidation , Methods of Detection, and Applications in Atherosclerosis Research.Methods in molecular biology (Clifton, N.J.) · 2022Article
- PCSK9 Functions in Atherosclerosis Are Not Limited to Plasmatic LDL-Cholesterol Regulation.Frontiers in cardiovascular medicine · 2021Review
- Plasma lipidomic biomarker analysis reveals distinct lipid changes in vascular dementia.Computational and structural biotechnology journal · 2020Article
- Peripheral Acid Sphingomyelinase Activity Is Associated with Biomarkers and Phenotypes of Alcohol Use and Dependence in Patients and Healthy Controls.International journal of molecular sciences · 2018Article
- Susceptibility of low-density lipoprotein particles to aggregate depends on particle lipidome, is modifiable, and associates with future cardiovascular deaths.European heart journal · 2018Article
- Secretory sphingomyelinase (S-SMase) activity is elevated in patients with rheumatoid arthritis.Clinical rheumatology · 2018Article
- Article
- Plasma cross-gestational sphingolipidomic analyses reveal potential first trimester biomarkers of preeclampsia.PloS one · 2017Article
- Sphingosine 1-Phosphate Activation of EGFR As a Novel Target for Meningitic Escherichia coli Penetration of the Blood-Brain Barrier.PLoS pathogens · 2016Article
- Alcohol and high fat induced chronic pancreatitis: TRPV4 antagonist reduces hypersensitivity.Neuroscience · 2015Article
- Increasing adipocyte lipoprotein lipase improves glucose metabolism in high fat diet-induced obesity.The Journal of biological chemistry · 2015Article
- ORMDL/serine palmitoyltransferase stoichiometry determines effects of ORMDL3 expression on sphingolipid biosynthesis.Journal of lipid research · 2015Article
- Ceramide as a mediator of non-alcoholic Fatty liver disease and associated atherosclerosis.PloS one · 2015Article
- Sphingomyelin in high-density lipoproteins: structural role and biological function.International journal of molecular sciences · 2013Review
- Metabolites and metabolic pathways associated with glucocorticoid resistance in pregnant African-American women.Comprehensive psychoneuroendocrinologyArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The propensity of LDLs (low-density lipoproteins) for aggregation and/or oxidation has been linked to their sphingolipid content, specifically the levels of SM (sphingomyelin) and ceramide. To investigate this association in vivo, ldlr (LDL receptor)-null mice (ldlr-/-) were fed on a modified (atherogenic) diet containing saturated fats and cholesterol. The diet led to significantly elevated SM content in all serum lipoproteins. In contrast, ceramide increased only in the LDL particles. MS-based analyses of the lipid acyl chain composition revealed a marked elevation in C16:0 fatty acid in SM and ceramide, consistent with the prevalence of palmitic acid in the modified diet. The diet also led to increased activity of the S-SMase [secretory SMase (sphingomyelinase)], a protein that is generated by ASMase (acid SMase) and acts on serum LDL. An increased macrophage secretion seemed to be responsible for the elevated S-SMase activity. ASMase-deficient mice (asm-/-/ldlr-/-) lacked S-SMase activity and were protected from diet-induced elevation in LDL ceramide. LDL from asm-/-/ldlr-/- mice fed on the modified diet were less aggregated and oxidized than LDL from asm+/+/ldlr-/- mice. When tested in vitro, the propensity for aggregation was dependent on the SM level: only LDL from animals on modified diet that have high SM content aggregated when treated with recombinant S-SMase. In conclusion, LDL-SM content and S-SMase activity are up-regulated in mice fed on an atherogenic diet. S-SMase mediates diet-induced changes in LDL ceramide content and aggregation. S-SMase effectiveness in inducing aggregation is dependent on diet-induced enrichment of LDL with SM, possibly through increased hepatic synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.