ArticleThe Journal of physiology2012
Mitochondrial activation at the onset of contractions in isolated myofibres during successive contractile periods.
Article in The Journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Effects of prior heavy exercise on deoxygenation in deep versus superficial muscle regions during subsequent heavy exercise in adult males.Physiological reports · 2026Article
- Absence of Parkin Results in Atrophy of Oxidative Myofibers and Modulation of AKT and MURF1 Signaling in Middle-Aged Male Mice.Acta physiologica (Oxford, England) · 2025Article
- Time-of-day effects on muscle mitochondria following short-term ablation of satellite cells.Frontiers in physiology · 2025Article
- How Priming Exercise Affects Oxygen Uptake Kinetics: From Underpinning Mechanisms to Endurance Performance.Sports medicine (Auckland, N.Z.) · 2023Review
- A mitochondrial-targeted antioxidant improves myofilament CaThe Journal of physiology · 2018Article
- Mitochondrial NAD(P)H In vivo: Identifying Natural Indicators of Oxidative Phosphorylation in the (31)P Magnetic Resonance Spectrum.Frontiers in physiology · 2016Article
- MRS Evidence of Adequate O₂ Supply in Human Skeletal Muscle at the Onset of Exercise.Medicine and science in sports and exercise · 2015Article
- Limitations to oxygen transport and utilization during sprint exercise in humans: evidence for a functional reserve in muscle O2 diffusing capacity.The Journal of physiology · 2015Article
- Combinatorial polymer matrices enhance in vitro maturation of human induced pluripotent stem cell-derived cardiomyocytes.Biomaterials · 2015Article
- Ischemic preconditioning accelerates muscle deoxygenation dynamics and enhances exercise endurance during the work-to-work test.Physiological reports · 2015Article
- Rapid changes in NADH and flavin autofluorescence in rat cardiac trabeculae reveal large mitochondrial complex II reserve capacity.The Journal of physiology · 2015Article
- Age affects the contraction-induced mitochondrial redox response in skeletal muscle.Frontiers in physiology · 2015Article
- Separation of metabolic supply and demand: aerobic glycolysis as a normal physiological response to fluctuating energetic demands in the membrane.Cancer & metabolism · 2014Article
- Bang-bang model for regulation of local blood flow.Microcirculation (New York, N.Y. : 1994) · 2013Review
- Effect of calcium on the oxidative phosphorylation cascade in skeletal muscle mitochondria.Biochemistry · 2013Article
- On-off asymmetries in oxygen consumption kinetics of single Xenopus laevis skeletal muscle fibres suggest higher-order control.The Journal of physiology · 2013Article
- Increasing temperature speeds intracellular PO2 kinetics during contractions in single Xenopus skeletal muscle fibers.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Article
- Mitochondrial creatine kinase activity and phosphate shuttling are acutely regulated by exercise in human skeletal muscle.The Journal of physiology · 2012Article
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Authors and funding
3 authors.
Funding
Abstract
At the onset of skeletal muscle repetitive contractions, there is a significant delay in the time to achieve oxidative phosphorylation steady state. The purpose of the present study was to examine the factors that limit oxidative phosphorylation at the onset of contractions. NAD(P)H was measured in real time during two contractile periods (2 min each) separated by 5 min of rest in intact single muscle fibres (n = 7) isolated from Xenopus laevis. The fibres were then loaded with the dye tetramethylrhodamine methyl ester perchlorate (TMRM) to evaluate the kinetics of the mitochondrial membrane potential (Δψ (m)) during two further successive contractile periods. At the onset of contractions in the first period, NAD(P)H exhibited a time delay (14.1 ± 1.3 s) before decreasing toward a steady state. In contrast, Δψ(m) decreased immediately after the first contraction and started to be reestablished after 10.7 ± 0.9 s, with restoration to the pre-stimulation values after approximately 32 s. In the second contractile period (5 min after the first), NAD(P)H decreased immediately (i.e. no time delay) after the first contraction and had a significantly shorter time constant compared to the first contractile bout (3.3 ± 0.3 vs. 5.0 ± 0.2 s, P < 0.05). During the second bout, Δψ(m) remained unchanged from pre-stimulation values. These results suggest: (1) that at the onset of contractions, oxidative phosphorylation is primarily limited by the activity of the electron transport chain complexes rather than by a limited level of substrates; and (2) when the muscle is 'primed' by previous contractile activity, the faster enhancement of the cellular respiratory rate is due to intrinsic factors within the myofibre.
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