ArticleProceedings of the National Academy of Sciences of the United States of America2012
Crown ether-electrolyte interactions permit nanopore detection of individual DNA abasic sites in single molecules.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed.
- Nanopore Environmental Analysis.JACS Au · 2025Review
- Investigating the origins of the mutational signatures in cancer.Nucleic acids research · 2025Review
- A nanopore interface for higher bandwidth DNA computing.Nature communications · 2022Article
- Products Generated by Amine-Catalyzed Strand Cleavage at Apurinic/Apyrimidinic Sites in DNA: New Insights from a Biomimetic Nucleoside Model System.Chemical research in toxicology · 2022Article
- Current and Future Methodology for Quantitation and Site-Specific Mapping the Location of DNA Adducts.Toxics · 2022Article
- Synthesis of DNA Duplexes Containing Site-Specific Interstrand Cross-Links via Sequential Reductive Amination Reactions Involving Diamine Linkers and Abasic Sites on Complementary Oligodeoxynucleotides.Chemical research in toxicology · 2021Article
- Detection and Discrimination of DNA Adducts Differing in Size, Regiochemistry, and Functional Group by Nanopore Sequencing.Chemical research in toxicology · 2020Article
- Next-Generation Genotoxicology: Using Modern Sequencing Technologies to Assess Somatic Mutagenesis and Cancer Risk.Environmental and molecular mutagenesis · 2020Review
- A nanofluidic ion regulation membrane with aligned cellulose nanofibers.Science advances · 2019Article
- Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites: 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine and 2'-Deoxycytidine.Methods in molecular biology (Clifton, N.J.) · 2019Article
- A Nanopore Phosphorylation Sensor for Single Oligonucleotides and Peptides.Research (Washington, D.C.) · 2019Article
- Single Locked Nucleic Acid-Enhanced Nanopore Genetic Discrimination of Pathogenic Serotypes and Cancer Driver Mutations.ACS nano · 2018Article
- What is the potential of nanolock- and nanocross-nanopore technology in cancer diagnosis?Expert review of molecular diagnostics · 2018Review
- Role of outer surface probes for regulating ion gating of nanochannels.Nature communications · 2018Article
- Nanolock-Nanopore Facilitated Digital Diagnostics of Cancer Driver Mutation in Tumor Tissue.ACS sensors · 2017Article
- Interrogation of Base Pairing of the Spiroiminodihydantoin Diastereomers Using the α-Hemolysin Latch.Biochemistry · 2017Article
- Polycationic Probe-Guided Nanopore Single-Molecule Counter for Selective miRNA Detection.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Kinetics of T3-DNA Ligase-Catalyzed Phosphodiester Bond Formation Measured Using the α-Hemolysin Nanopore.ACS nano · 2016Article
- Human Telomere G-Quadruplexes with Five Repeats Accommodate 8-Oxo-7,8-dihydroguanine by Looping out the DNA Damage.ACS chemical biology · 2016Article
- Sequencing of DNA Lesions Facilitated by Site-Specific Excision via Base Excision Repair DNA Glycosylases Yielding Ligatable Gaps.Journal of the American Chemical Society · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
DNA abasic (AP) sites are one of the most frequent lesions in the genome and have a high mutagenic potential if unrepaired. After selective attachment of 2-aminomethyl-18-crown-6 (18c6), individual AP lesions are detected during electrophoretic translocation through the bacterial protein ion channel α-hemolysin (α-HL) embedded in a lipid bilayer. Interactions between 18c6 and Na(+) produce characteristic pulse-like current amplitude signatures that allow the identification of individual AP sites in single molecules of homopolymeric or heteropolymeric DNA sequences. The bulky 18c6-cation complexes also dramatically slow the DNA motion to more easily recordable levels. Further, the behaviors of the AP-18c6 adduct are different with respect to the directionalities of DNA entering the protein channel, and they can be precisely manipulated by altering the cation (Li(+), Na(+) or K(+)) of the electrolyte. This method permits detection of multiple AP lesions per strand, which is unprecedented in other work. Additionally, insights into the thermodynamics and kinetics of 18c6-cation interactions at a single-molecule level are provided by the nanopore measurement.
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