ArticlePlant physiology2012
Extending cassava root shelf life via reduction of reactive oxygen species production.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 161 citations in OpenAlex.
- Genome-wide association study of post-harvest physiological deterioration in cassava (Frontiers in plant science · 2026Article
- Progress and Impacts of "Omics" Technologies in Understanding the Drought Response in Cassava: Adoption for Food Security in Africa.Plant-environment interactions (Hoboken, N.J.) · 2025Review
- Advancements and strategies of genetic improvement in cassava (Horticulture research · 2025Article
- Systematic Analysis of Cinnamyl Alcohol Dehydrogenase Family in Cassava and Validation ofInternational journal of molecular sciences · 2024Article
- Constitutive overexpression ofFrontiers in plant science · 2024Article
- The role of small RNAs in resistant melon cultivar againstFrontiers in microbiology · 2024Article
- Superoxide dismutase gene family in cassava revealed their involvement in environmental stress via genome-wide analysis.iScience · 2023Article
- Reduced glutathione and raffinose lengthens postharvest storage of cassava root tubers by improving antioxidant capacity and antibiosis.BMC plant biology · 2023Article
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- CRISPR/Cas9: an advanced platform for root and tuber crops improvement.Frontiers in genome editing · 2023Review
- Reactive oxygen species turnover, phenolics metabolism, and some key gene expressions modulate postharvest physiological deterioration in cassava tubers.Frontiers in microbiology · 2023Article
- Knockdown ofInternational journal of molecular sciences · 2022Article
- Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration.Plant molecular biology · 2022Article
- Oxidative Stress and DNA Damage Effect ofMolecules (Basel, Switzerland) · 2022Article
- A promoter toolbox for tissue-specific expression supporting translational research in cassava (Frontiers in plant science · 2022Article
- CRISPR-Cas9-mediated knockout ofFrontiers in plant science · 2022Article
- Systematic Analysis of bHLH Transcription Factors in Cassava Uncovers Their Roles in Postharvest Physiological Deterioration and Cyanogenic Glycosides Biosynthesis.Frontiers in plant science · 2022Article
- Carbohydrate Partitioning and Antioxidant Substances Synthesis Clarify the Differences Between Sugarcane Varieties on Facing Low Phosphorus Availability.Frontiers in plant science · 2022Article
- Genome-wide survey of the phosphofructokinase family in cassava and functional characterization in response to oxygen-deficient stress.BMC plant biology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the major constraints facing the large-scale production of cassava (Manihot esculenta) roots is the rapid postharvest physiological deterioration (PPD) that occurs within 72 h following harvest. One of the earliest recognized biochemical events during the initiation of PPD is a rapid burst of reactive oxygen species (ROS) accumulation. We have investigated the source of this oxidative burst to identify possible strategies to limit its extent and to extend cassava root shelf life. We provide evidence for a causal link between cyanogenesis and the onset of the oxidative burst that triggers PPD. By measuring ROS accumulation in transgenic low-cyanogen plants with and without cyanide complementation, we show that PPD is cyanide dependent, presumably resulting from a cyanide-dependent inhibition of respiration. To reduce cyanide-dependent ROS production in cassava root mitochondria, we generated transgenic plants expressing a codon-optimized Arabidopsis (Arabidopsis thaliana) mitochondrial alternative oxidase gene (AOX1A). Unlike cytochrome c oxidase, AOX is cyanide insensitive. Transgenic plants overexpressing AOX exhibited over a 10-fold reduction in ROS accumulation compared with wild-type plants. The reduction in ROS accumulation was associated with a delayed onset of PPD by 14 to 21 d after harvest of greenhouse-grown plants. The delay in PPD in transgenic plants was also observed under field conditions, but with a root biomass yield loss in the highest AOX-expressing lines. These data reveal a mechanism for PPD in cassava based on cyanide-induced oxidative stress as well as PPD control strategies involving inhibition of ROS production or its sequestration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.