ArticleThe Journal of cell biology2012
Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis.
Article in The Journal of cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- The cytokinetic midbody mediates asymmetric fate specification at mitotic exit during neural stem cell division.bioRxiv : the preprint server for biology · 2024Article
- Fission-independent compartmentalization of mitochondria during budding yeast cell division.The Journal of cell biology · 2024Article
- Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources.iScience · 2023Article
- Quantitative analysis of nuclear pore complex organization inLife science alliance · 2022Article
- DNA circles promote yeast ageing in part through stimulating the reorganization of nuclear pore complexes.eLife · 2022Article
- Article
- Asymmetric nuclear division in neural stem cells generates sibling nuclei that differ in size, envelope composition, and chromatin organization.Current biology : CB · 2021Article
- Cellular quality control during gametogenesis.Experimental cell research · 2020Review
- Article
- The dynamic nuclear periphery as a facilitator of gamete health and rejuvenation.Current genetics · 2020Review
- Compositional reorganization of the nucleolus in budding yeast mitosis.Molecular biology of the cell · 2019Article
- Spatial cues and not spindle pole maturation drive the asymmetry of astral microtubules between new and preexisting spindle poles.Molecular biology of the cell · 2018Article
- Article
- The Unsolved Problem of How Cells Sense Micron-Scale Curvature.Trends in biochemical sciences · 2017Review
- Budding yeast Wee1 distinguishes spindle pole bodies to guide their pattern of age-dependent segregation.Nature cell biology · 2017Article
- Article
- High resolution microscopy reveals the nuclear shape of budding yeast during cell cycle and in various biological states.Journal of cell science · 2016Article
- Compartmentalization of the endoplasmic reticulum in the early C. elegans embryos.The Journal of cell biology · 2016Article
- Mixing and matching nuclear envelope remodeling and spindle assembly strategies in the evolution of mitosis.Current opinion in cell biology · 2016Review
- Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly.The Journal of cell biology · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
Abstract
During vegetative growth, Saccharomyces cerevisiae cells divide asymmetrically: the mother cell buds to produce a smaller daughter cell. This daughter asymmetrically inherits the transcription factor Ace2, which activates daughter-specific transcriptional programs. In this paper, we investigate when and how this asymmetry is established and maintained. We show that Ace2 asymmetry is initiated in the elongated, but undivided, anaphase nucleus. At this stage, the nucleoplasm was highly compartmentalized; little exchange was observed for nucleoplasmic proteins between mother and bud. Using photobleaching and in silico modeling, we show that diffusion barriers compartmentalize the nuclear membranes. In contrast, the behavior of proteins in the nucleoplasm is well explained by the dumbbell shape of the anaphase nucleus. This compartmentalization of the nucleoplasm promoted Ace2 asymmetry in anaphase nuclei. Thus, our data indicate that yeast cells use the process of closed mitosis and the morphological constraints associated with it to asymmetrically segregate nucleoplasmic components.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.