ArticleEcotoxicology (London, England)2012
A novel metallothionein gene from a mangrove plant Kandelia candel.
Article in Ecotoxicology (London, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 22 citations in OpenAlex.
- Cloning and Expression of Class I Chitinase Genes from Four Mangrove Species under Heavy Metal Stress.Plants (Basel, Switzerland) · 2023Article
- Molecular Cloning and Expression Analysis of the Typical Class III Chitinase Genes from Three Mangrove Species under Heavy Metal Stress.Plants (Basel, Switzerland) · 2023Article
- In silico analysis of NAC gene family in the mangrove plant Avicennia marina provides clues for adaptation to intertidal habitats.Plant molecular biology · 2023Article
- Genetic and molecular mechanisms underlying mangrove adaptations to intertidal environments.iScience · 2022Review
- A comparison of 25 complete chloroplast genomes between sister mangrove speciesFrontiers in plant science · 2022Article
- Study on metal binding capacity of the freshwater crab Sinopotamon henanense's recombinant copper specific binding metallothionein expressed in Escherichia coli.Ecotoxicology (London, England) · 2022Article
- Impact of Silicon Nanoparticles on the Antioxidant Compounds of Tomato Fruits Stressed by Arsenic.Foods (Basel, Switzerland) · 2019Article
- Pb uptake and tolerance in the two selected mangroves with different root lignification and suberization.Ecotoxicology (London, England) · 2015Article
- Performance of the subsurface flow constructed wetlands for pretreatment of slightly polluted source water.Ecotoxicology (London, England) · 2014Article
- Molecular cloning and characterisation of metallothionein type 2a gene from Jatropha curcas L., a promising biofuel plant.Molecular biology reports · 2014Article
- Expression of metallothionein of freshwater crab (Sinopotamon henanense) in Escherichia coli enhances tolerance and accumulation of zinc, copper and cadmium.Ecotoxicology (London, England) · 2014Article
- cDNA sequence encoding metallothionein protein from Aegiceras corniculatum and its gene expression induced by Pb²⁺ and Cd²⁺ stresses.Environmental monitoring and assessment · 2013Article
- Protective role of hydrogen peroxide pretreatment on defense systems and BnMP1 gene expression in Cr(VI)-stressed canola seedlings.Ecotoxicology (London, England) · 2013Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new metallothionein (MT) gene was cloned from Kandelia candel, a mangrove plant with constitutional tolerance to heavy metals, by rapid amplification of cDNA ends and named KMT, which is composed of two exons and one intron. The full length of KMT cDNA was 728 bp including 121 bp 5' noncoding domain, 240 bp open reading frame and 384 bp 3' termination. The coding region of KMT represented a putative 79 amino acid protein with a molecular weight of 7.75 kDa. At each of the amino- and carboxy-terminal of the putative protein, cysteine residues were arranged in Cys-Cys, Cys-X-Cys and Cys-X-X-Cys, indicating that the putative protein was a novel type 2 MT. Sequence and homology analysis showed the KMT protein sequence shared more than 60 % homology with other plant type 2 MT-like protein genes. At amino acid level, the KMT was shown homology with the MT of Quercus suber (83 %), of Ricinus communis (81 %) and of Arabidopsis thaliana (64 %). Function studies using protease-deficient Escherichia coli strain BL21 Star ™(DE3) confirmed the functional nature of this KMT gene in sequestering both essential (Zn) and non-essential metals (Cd and Hg) and the E. coli BL21 with KMT can live in 1,000 μmol/L Zn, 120 μmol/L Hg, and 2,000 μmol/L Cd. The information could provide more details of the causative molecular and biochemical mechanisms (including heavy metal sequestration) of the KMT in K. candel or a scientific basis for marine heavy-metal environment remediation with K. candel. This study also provides a great significance of protecting mangrove species and mangrove ecosystem.
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