ArticleJournal of visualized experiments : JoVE2012
Ovariectomy and 17β-estradiol replacement in rats and mice: a visual demonstration.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
94 citing papers in PubMed, 1 synthesis or guideline pooled it, 116 citations in OpenAlex.
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- Oestrogen modulates stress-induced vagally-dependent gastric responses to oxytocin.The Journal of physiology · 2026Article
- Oestrogen receptors regulate vascular β₁- and β₃-adrenoceptor expression and shape sex-specific adrenergic control of vascular tone.Pflugers Archiv : European journal of physiology · 2026Article
- A rise in prepubertal DHEA in the spiny mouse reveals the effect of adrenarche on the reproductive axis.BMC biology · 2026Article
- Ovarian hormones moderate systolic hypertension in femaleAmerican journal of physiology. Renal physiology · 2026Article
- CD4Nature communications · 2026Article
- Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF.iScience · 2026Article
- Article
- Effects of Sex, Estradiol, and Acute Stress on Spontaneous Recovery of a Conditioned Touchscreen Response in Rats.Neuroscience insights · 2026Article
- Sex-dependent protective responses of taurine in ameliorating radiation-induced intestinal injury: gut microbiota reprogramming in males and estrogen-dependent immunity in females.International journal of biological sciences · 2026Article
- Ovarian Hormones Moderate Systolic Hypertension in FemalebioRxiv : the preprint server for biology · 2025Article
- Sexual dimorphism of lung immune-regulatory units imprint biased pulmonary fibrosis.Cellular & molecular immunology · 2025Article
- One size does not fit all: how type of menopause and hormone therapy matters for brain health.The British journal of psychiatry : the journal of mental science · 2025Review
- A circadian behavioral analysis suite for real-time classification of daily rhythms in complex behaviors.Cell reports methods · 2025Article
- The effects of pharmacologic estradiol on anxiety-related behavior in adolescent and adult female mice.Physiology & behavior · 2025Article
- The Role of Estrogen and ER Stress in Glycemic Regulation in the Sexually Dimorphic TALLYHO/JngJ Mouse Model of Diabetes.Journal of the Endocrine Society · 2025Article
- The crucial role of beta-catenin in the osteoprotective effect of semaglutide in an ovariectomized rat model of osteoporosis.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
- Sex Chromosomes and Sex Hormones: Dissecting the Forces That Differentiate Female and Male Hearts.Circulation · 2025Review
- Guidelines for sex-specific considerations to improve rigor in renal research and how we got there.American journal of physiology. Renal physiology · 2025Review
34 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Estrogens are a family of female sexual hormones with an exceptionally wide spectrum of effects. When rats and mice are used in estrogen research they are commonly ovariectomized in order to ablate the rapidly cycling hormone production, replacing the 17β-estradiol exogenously. There is, however, lack of consensus regarding how the hormone should be administered to obtain physiological serum concentrations. This is crucial since the 17β-estradiol level/administration method profoundly influences the experimental results. We have in a series of studies characterized the different modes of 17β-estradiol administration, finding that subcutaneous silastic capsules and per-oral nut-cream Nutella are superior to commercially available slow-release pellets (produced by the company Innovative Research of America) and daily injections in terms of producing physiological serum concentrations of 17β-estradiol. Amongst the advantages of the nut-cream method, that previously has been used for buprenorphine administration, is that when used for estrogen administration it resembles peroral hormone replacement therapy and is non-invasive. The subcutaneous silastic capsules are convenient and produce the most stable serum concentrations. This video article contains step-by-step demonstrations of ovariectomy and 17β-estradiol hormone replacement by silastic capsules and peroral Nutella in rats and mice, followed by a discussion of important aspects of the administration procedures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.