ArticleMicrobial cell factories2012
Achieving efficient protein expression in Trichoderma reesei by using strong constitutive promoters.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 118 citations in OpenAlex.
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- Heterologously Expressed Cellobiose Dehydrogenase Acts as Efficient Electron-Donor of Lytic Polysaccharide Monooxygenase for Cellulose Degradation inInternational journal of molecular sciences · 2023Article
- A multi-plex protein expression system for production of complex enzyme formulations in Trichoderma reesei.Journal of industrial microbiology & biotechnology · 2023Article
- Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei.Bioresources and bioprocessing · 2022Article
- Heterologous Expression of Secondary Metabolite Genes inJournal of fungi (Basel, Switzerland) · 2022Article
- Simultaneous manipulation of multiple genes within a same regulatory stage for iterative evolution of Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2022Article
- A sensitive, accurate, and high-throughput gluco-oligosaccharide oxidase-based HRP colorimetric method for assaying lytic polysaccharide monooxygenase activity.Biotechnology for biofuels and bioproducts · 2022Article
- Development of a powerful synthetic hybrid promoter to improve the cellulase system of Trichoderma reesei for efficient saccharification of corncob residues.Microbial cell factories · 2022Article
- An overview on current molecular tools for heterologous gene expression in Trichoderma.Fungal biology and biotechnology · 2021Review
- Article
- Rational engineering of xylanase hyper-producing system in Trichoderma reesei for efficient biomass degradation.Biotechnology for biofuels · 2021Article
- Optimization of Cephalosporin C Acylase Expression in Escherichia coli by High-Throughput Screening a Constitutive Promoter Mutant library.Applied biochemistry and biotechnology · 2021Article
- Securing a furan-based biorefinery: disclosing the genetic basis of the degradation of hydroxymethylfurfural and its derivatives in the model fungus Aspergillus nidulans.Microbial biotechnology · 2020Article
- Assessment of the ptxD gene as a growth and selective marker in Trichoderma atroviride using Pccg6, a novel constitutive promoter.Microbial cell factories · 2020Article
- Improvement of Saccharification and Delignification Efficiency ofMicroorganisms · 2020Article
- Rational engineering of theBiotechnology for biofuels · 2020Article
- Interfacial molecular interactions of cellobiohydrolase Cel7A and its variants on cellulose.Biotechnology for biofuels · 2020Article
- Novel genetic tools that enable highly pure protein production in Trichoderma reesei.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundsThe fungus Trichoderma reesei is an important workhorse for expression of homologous or heterologous genes, and the inducible cbh1 promoter is generally used. However, constitutive expression is more preferable in some cases than inducible expression that leads to production of unwanted cellulase components. In this work, constitutive promoters of T. reesei were screened and successfully used for high level homologous expression of xylanase II.
resultsThe transcriptional profiles of 13 key genes that participate in glucose metabolism in T. reesei were analyzed by quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR). The results indicated that the mRNA levels of pdc (encoding pyruvate decarboxylase) and eno (encoding enolase) genes were much higher than other genes under high glucose conditions. Recombinant T. reesei strains that homologously expressed xylanase II were constructed by using the promoters of the pdc and eno genes, and they respectively produced 9266 IU/ml and 8866 IU/ml of xylanase activities in the cultivation supernatant in a medium with high glucose concentration. The productivities of xylanase II were 1.61 g/L (with the pdc promoter) and 1.52 g/L (with the eno promoter), approximately accounted for 83% and 82% of the total protein secreted by T. reesei, respectively.
conclusionsThis work demonstrates the screening of constitutive promoters by using RT-qPCR in T. reesei, and has obtained the highest expression of recombinant xylanase II to date by using these promoters.
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