Evidence map›Paper›PMID 22708072›Full record

ArticleJournal of biomaterials and nanobiotechnology2012

Transparent Electrode Materials for Simultaneous Amperometric Detection of Exocytosis and Fluorescence Microscopy.

Kassandra Kisler, Brian N Kim, Xin Liu, Khajak Berberian, Qinghua Fang, Cherian J Mathai, Shubhra Gangopadhyay, Kevin D Gillis, Manfred Lindau

Open access · diamondAbstract read
In one paragraph

Article in Journal of biomaterials and nanobiotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 47 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Electrochemical measurement of quantal exocytosis using microchips.Pflugers Archiv : European journal of physiology · 2018
    Review
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  10. Article
  11. Article
  12. Article
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  14. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Kassandra KislerSchool of Applied and Engineering Physics, Cornell University, Ithaca, NY USA.
Brian N Kim
Xin Liu
Khajak Berberian
Qinghua Fang
Cherian J Mathai
Shubhra Gangopadhyay
Kevin D Gillis
Manfred Lindau
University of Missouri · USCornell University · USMax Planck Institute for Biophysical Chemistry · DE

Funding

TRAINING IN MOLECULAR PHYSICS OF BIOLOGICAL SYSTEMST32GM008267 · NIGMS · CORNELL UNIVERSITY ITHACA · PI WANG, MICHELLE D. · 1988 to 2021
$9.6M
Microchip devices to assay quantal exocytosisR01NS048826 · NINDS · UNIVERSITY OF MISSOURI-COLUMBIA · PI GILLIS, KEVIN D · 2004 to 2009
$2.8M
CELLULAR AND MOLECULAR NEUROBIOLOGYT32GM007469 · NIGMS · CORNELL UNIVERSITY ITHACA · PI HARRIS-WARRICK, RONALD M · 1985 to 2011
$2.5M
Mechanisms of Fusion &Transmitter releaseR01NS038200 · NINDS · CORNELL UNIVERSITY ITHACA · PI LINDAU, MANFRED · 2000 to 2007
$2.3M
Mechanisms of fusion and transmitter release in neurosecretionR01GM085808 · NIGMS · CORNELL UNIVERSITY · PI LINDAU, MANFRED · 2008 to 2012
$1.7M
Scalable sensor array platform for analysis of quantal transmitter release eventsR01MH095046 · NIMH · CORNELL UNIVERSITY · PI LINDAU, MANFRED · 2011 to 2014
$1.5M
NIGMS NIH HHS R01 GM085808NIGMS NIH HHS T32 GM007469NIGMS NIH HHS T32 GM008267NIMH NIH HHS R01 MH095046NINDS NIH HHS R01 NS038200NINDS NIH HHS R01 NS048826
6 · The paper itself

Abstract

We have developed and tested transparent microelectrode arrays capable of simultaneous amperometric measurement of oxidizable molecules and fluorescence imaging through the electrodes. Surface patterned microelectrodes were fabricated from three different conducting materials: Indium-tin-oxide (ITO), nitrogen-doped diamond-like carbon (DLC) deposited on top of ITO, or very thin (12-17 nm) gold films on glass substrates. Chromaffin cells loaded with lysotracker green or acridine orange dye were placed atop the electrodes and vesicle fluorescence imaged with total internal reflection fluorescence (TIRF) microscopy while catecholamine release from single vesicles was measured as amperometric spikes with the surface patterned electrodes. Electrodes fabricated from all three materials were capable of detecting amperometric signals with high resolution. Unexpectedly, amperometric spikes recorded with ITO electrodes had only about half the amplitude and about half as much charge as those detected with DLC or gold electrodes, indicating that the ITO electrodes are not as sensitive as gold or DLC electrodes for measurement of quantal catecholamine release. The lower sensitivity of ITO electrodes was confirmed by chronoamperometry measurements comparing the currents in the presence of different analytes with the different electrode materials.

Identifiers

PMID22708072
PMCPMC3375995
OpenAlexW2083810544

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.