ArticlePsychiatry investigation2012
Fluoxetine increases the expression of NCAM140 and pCREB in rat C6 glioma cells.
Article in Psychiatry investigation, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 6 citations in OpenAlex.
- RETRACTED: Duloxetine, an SNRI, Targets pSTAT3 Signaling:International journal of molecular sciences · 2025Article
- Fluoxetine Increases the Expression of miR-572 and miR-663a in Human Neuroblastoma Cell Lines.PloS one · 2016Article
- Profiling of Proteins Regulated by Venlafaxine during Neural Differentiation of Human Cells.Psychiatry investigation · 2015Article
- NCAM140 and pCREB Expression after Tianeptine Treatment of SH-SY5Y Cells.Psychiatry investigation · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveDysfunction of neural plasticity in the brain is known to alter neural networks, resulting in depression. To understand how fluoxetine regulates molecules involved in neural plasticity, the expression levels of NCAM, NCAM140, CREB and pCREB, in rat C6 glioma cells after fluoxetine treatment were examined.
methodsC6 cells were cultured after 20 min or after 6, 24 or 72 h treatments with 10 µM fluoxetine. Immunocytochemistry was used to determine the effect of fluoxetine on the expression of NCAM. Western blot analysis was used to measure the expression levels of NCAM140 and CREB and the induction of pCREB after fluoxetine treatment.
resultsNCAM expression following 72-h fluoxetine treatment was significantly increased around cell membranes compared to control cells. Cells treated with fluoxetine for 6 and 72 h showed a significant increase in NCAM140 expression compared to cells treated for 20 min. The level of pCREB in the cells treated with fluoxetine for 72 h not only increased more than 60%, but was also significantly different when compared with the other treatment times. The 72-h fluoxetine treatment led to the increase of NCAM140 and the phosphorylation of CREB in C6 cells.
conclusionOur findings indicate that fluoxetine treatment regulates neuronal plasticity and neurite outgrowth by phosphorylating and activating CREB via the NCAM140 homophilic interaction-induced activation of the Ras-MAPK pathway.
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