ArticleFrontiers in microbiology2012
Versatile Genetic Tool Box for the Crenarchaeote Sulfolobus acidocaldarius.
Article in Frontiers in microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 133 papers.
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133 citing papers in PubMed, 216 citations in OpenAlex.
- Review
- OTTR-seq profiling reveals dynamic tRNA modification landscapes across diverse archaeal species.Genome biology · 2026Article
- The mechanism of cell-cycle-dependent proteasomal degradation of archaeal ESCRT-III homolog CdvB in Sulfolobus.The EMBO journal · 2026Article
- Article
- Removal and bypass of restriction modification systems increases transformation efficiency ofFrontiers in microbiology · 2026Article
- In Vivo RNA Chemical Footprinting Analysis in Archaea.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Temporal and spatial coordination of DNA segregation and cell division in an archaeon.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Landscape of tRNA Modifications in Archaea.bioRxiv : the preprint server for biology · 2025Article
- A temperature-sensitive mutant screen reveals a translational stress-induced cell-cycle arrest in a thermophilic archaeon.Molecular biology of the cell · 2025Article
- Coupling chromosome organization to genome segregation in Archaea.Nature communications · 2025Article
- A self-assembling surface layer flattens the cytokinetic furrow to aid cell division in an archaeon.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Archaeal SegAB forms a bipolar structure that promotes chromosome segregation in spherical cells.bioRxiv : the preprint server for biology · 2025Article
- Cyclization of archaeal membrane lipids impacts membrane protein activity and archaellum formation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lipid hydrogen isotope compositions primarily reflect growth water in the model archaeonApplied and environmental microbiology · 2025Article
- An unusual glycerol-3-phosphate dehydrogenase in Sulfolobus acidocaldarius elucidates the diversity of glycerol metabolism across Archaea.Communications biology · 2025Article
- Bilayer-Forming Lipids Enhance Archaeal Monolayer Membrane Stability.International journal of molecular sciences · 2025Article
- Regulation of DNA Topology in Archaea: State of the Art and Perspectives.Molecular microbiology · 2025Review
- Article
- Effects of genomic location on ectopic integration and gene expression of a reporter gene cassette inFrontiers in microbiology · 2025Article
- Phosphorylation-driven conformational switching of the ArnA-ArnB complex involved in archaeal motility regulation.Frontiers in microbiology · 2025Article
73 more citing papers are in PubMed but not listed here.
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7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For reverse genetic approaches inactivation or selective modification of genes are required to elucidate their putative function. Sulfolobus acidocaldarius is a thermoacidophilic Crenarchaeon which grows optimally at 76°C and pH 3. As many antibiotics do not withstand these conditions the development of a genetic system in this organism is dependent on auxotrophies. Therefore we constructed a pyrE deletion mutant of S. acidocaldarius wild type strain DSM639 missing 322 bp called MW001. Using this strain as the starting point, we describe here different methods using single as well as double crossover events to obtain markerless deletion mutants, tag genes genomically and ectopically integrate foreign DNA into MW001. These methods enable us to construct single, double, and triple deletions strains that can still be complemented with the pRN1 based expression vector. Taken together we have developed a versatile and robust genetic tool box for the crenarchaeote S. acidocaldarius that will promote the study of unknown gene functions in this organism and makes it a suitable host for synthetic biology approaches.
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