ArticleThe Journal of biological chemistry2012
Structural features affecting trafficking, processing, and secretion of Trypanosoma cruzi mucins.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 31 citations in OpenAlex.
- Free GPIs and Comparison of GPI Structures Among Species.International journal of molecular sciences · 2025Review
- A divide-and-conquer approach to uncover the genomic structure of the highly virulent RA strain of Trypanosoma cruzi.Scientific reports · 2025Article
- An algorithm for annotation and classification of T. cruzi MASP sequences: towards a better understanding of the parasite genetic variability.BMC genomics · 2025Article
- Review
- Deep serological profiling of the Trypanosoma cruzi TSSA antigen reveals different epitopes and modes of recognition by Chagas disease patients.PLoS neglected tropical diseases · 2023Article
- Review
- Mechanisms Associated withLife (Basel, Switzerland) · 2021Review
- Serological Approaches for Trypanosoma cruzi Strain Typing.Trends in parasitology · 2021Review
- All Roads Lead to Cytosol:Frontiers in cellular and infection microbiology · 2021Review
- Parasite-host glycan interactions during Trypanosoma cruzi infection: trans-Sialidase rides the show.Biochimica et biophysica acta. Molecular basis of disease · 2020Review
- Signal peptide recognition in Trypanosoma cruzi GP82 adhesin relies on its localization at protein N-terminus.Scientific reports · 2019Article
- Trypanosoma cruzi surface mucins are involved in the attachment to the Triatoma infestans rectal ampoule.PLoS neglected tropical diseases · 2019Article
- Molecular and antigenic characterization of Trypanosoma cruzi TolT proteins.PLoS neglected tropical diseases · 2019Article
- Mucins and Pathogenic Mucin-Like Molecules Are Immunomodulators During Infection and Targets for Diagnostics and Vaccines.Frontiers in chemistry · 2019Review
- Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi trans-Sialidase.Methods in molecular biology (Clifton, N.J.) · 2019Article
- High-resolution profiling of linear B-cell epitopes from mucin-associated surface proteins (MASPs) of Trypanosoma cruzi during human infections.PLoS neglected tropical diseases · 2017Article
- The Trypomastigote Small Surface Antigen (TSSA) regulates Trypanosoma cruzi infectivity and differentiation.PLoS neglected tropical diseases · 2017Article
- The Trypanosoma cruzi Surface, a Nanoscale Patchwork Quilt.Trends in parasitology · 2017Review
- A Brief View of the Surface Membrane Proteins fromJournal of parasitology research · 2017Review
- Interactions between Trypanosoma cruzi Secreted Proteins and Host Cell Signaling Pathways.Frontiers in microbiology · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trypanosoma cruzi is wrapped by a dense coat of mucin-type molecules encoded by complex gene families termed TcSMUG and TcMUC, which are expressed in the insect- and mammal-dwelling forms of the parasite, respectively. Here, we dissect the contribution of distinct post-translational modifications on the trafficking of these glycoconjugates. In vivo tracing and characterization of tagged-variants expressed by transfected epimastigotes indicate that although the N-terminal signal peptide is responsible for targeting TcSMUG products to the endoplasmic reticulum (ER), the glycosyl phosphatidylinositol (GPI)-anchor likely functions as a forward transport signal for their timely progression along the secretory pathway. GPI-minus variants accumulate in the ER, with only a minor fraction being ultimately released to the medium as anchorless products. Secreted products, but not ER-accumulated ones, display several diagnostic features of mature mucin-type molecules including extensive O-type glycosylation, Galf-based epitopes recognized by monoclonal antibodies, and terminal Galp residues that become readily sialylated upon addition of parasite trans-sialidases. Processing of N-glycosylation site(s) is dispensable for the overall TcSMUG mucin-type maturation and secretion. Despite undergoing different O-glycosylation elaboration, TcMUC reporters yielded quite similar results, thus indicating that (i) molecular trafficking signals are structurally and functionally conserved between mucin families, and (ii) TcMUC and TcSMUG products are recognized and processed by a distinct repertoire of stage-specific glycosyltransferases. Thus, using the fidelity of a homologous expression system, we have defined some biosynthetic aspects of T. cruzi mucins, key molecules involved in parasite protection and virulence.
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