ArticleJournal of industrial microbiology & biotechnology2012
Fungal β-glucosidase expression in Saccharomyces cerevisiae.
Article in Journal of industrial microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 44 citations in OpenAlex.
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- Heterologous Expression of Plantaricin 423 and Mundticin ST4SA in Saccharomyces cerevisiae.Probiotics and antimicrobial proteins · 2024Article
- Towards an understanding of the enzymatic degradation of complex plant mannan structures.World journal of microbiology & biotechnology · 2023Review
- Constructing recombinant Saccharomyces cerevisiae strains for malic-to-fumaric acid conversion.FEMS microbiology letters · 2023Article
- Promoter-proximal introns impact recombinant amylase expression in Saccharomyces cerevisiae.FEMS yeast research · 2023Article
- Observation of Cellodextrin Accumulation Resulted from Non-Conventional Secretion of Intracellular β-Glucosidase by EngineeredJournal of microbiology and biotechnology · 2021Article
- Heterologous secretory expression of β-glucosidase from Thermoascus aurantiacus in industrial Saccharomyces cerevisiae strains.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Construction of industrialBiotechnology for biofuels · 2019Article
- The heterologous expression potential of an acid-tolerant Talaromyces pinophilus β-glucosidase in Saccharomyces cerevisiae.Folia microbiologica · 2018Article
- Biocontrol Ability and Action Mechanism of Starmerella bacillaris (Synonym Candida zemplinina) Isolated from Wine Musts against Gray Mold Disease Agent Botrytis cinerea on Grape and Their Effects on Alcoholic Fermentation.Frontiers in microbiology · 2016Article
- Molecular cloning and expression of thermostable glucose-tolerant β-glucosidase of Penicillium funiculosum NCL1 in Pichia pastoris and its characterization.Journal of industrial microbiology & biotechnology · 2015Article
- Expression and evaluation of enzymes required for the hydrolysis of galactomannan.Journal of industrial microbiology & biotechnology · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinant Saccharomyces cerevisiae strains expressing β-glucosidases from Thermoascus aurantiacus (Tabgl1) and Phanerochaete chrysosporium (PcbglB and Pccbgl1) were constructed and compared to S. cerevisiae Y294[SFI], previously identified as the best β-glucosidase-producing strain. The PcbglB was also intracellularly expressed in combination with the lac12 lactose permease of Kluyveromyces lactis in S. cerevisiae Y294[PcbglB + Lac12]. The recombinant extracellular β-glucosidases indicated maximum activity in the pH range 4-5 and temperature optima varying from 50 to 75 °C. The S. cerevisiae Y294[Pccbgl1] strain performed best under aerobic and anaerobic conditions, producing 2.6 times more β-glucosidase activity than S. cerevisiae Y294[SFI] and an ethanol concentration of 4.8 g l(-1) after 24 h of cultivation on cellobiose as sole carbohydrate source. S. cerevisiae Y294[Tabgl1] was unable to grow on cellobiose (liquid medium), whereas S. cerevisiae Y294[PcbglB + Lac12] exhibited limited growth.
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