ArticleAmerican journal of clinical pathology2012
Rapid detection and quantitation of BRAF mutations in hairy cell leukemia using a sensitive pyrosequencing assay.
Article in American journal of clinical pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- BRAF V600E mutation detection in hairy cell leukemia-utility of archival DNA from bone marrow aspirate/imprint smear and amplification refractory mutation system.Molecular biology reports · 2020Article
- Real-time detection of BRAF V600E mutation from archival hairy cell leukemia FFPE tissue by nanopore sequencing.Molecular biology reports · 2018Article
- Langerhans cell histiocytosis associated with lymphoma: an incidental finding that is not associated with BRAF or MAP2K1 mutations.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2017Article
- Potential role of AKT/mTOR signalling proteins in hairy cell leukaemia: association with BRAF/ERK activation and clinical outcome.Scientific reports · 2016Article
- BRAF V600E and MAP2K1 mutations in hairy cell leukemia and splenic marginal zone lymphoma cases.Annals of laboratory medicine · 2015Article
- BRAF Mutation in Hairy Cell Leukemia.Oncology reviews · 2014Review
- Update on the biology and treatment options for hairy cell leukemia.Current treatment options in oncology · 2014Review
- BRAF-V600E expression in precursor versus differentiated dendritic cells defines clinically distinct LCH risk groups.The Journal of experimental medicine · 2014Article
- Next-generation sequencing-based multigene mutational screening for acute myeloid leukemia using MiSeq: applicability for diagnostics and disease monitoring.Haematologica · 2014Article
- Hairy cell leukemia: short review, today's recommendations and outlook.Blood cancer journal · 2014Article
- Immunohistochemical analysis using a BRAF V600E mutation specific antibody is highly sensitive and specific for the diagnosis of hairy cell leukemia.International journal of clinical and experimental pathology · 2014Article
- Hairy cell leukemia-new genes, new targets.Current hematologic malignancy reports · 2013Review
- Shifted termination assay (STA) fragment analysis to detect BRAF V600 mutations in papillary thyroid carcinomas.Diagnostic pathology · 2013Article
- Discrepant results for BRAF V600E mutation among different specimens in a case of hairy cell leukemia.Annals of laboratory medicine · 2013Article
- Frequency and spectrum of BRAF mutations in a retrospective, single-institution study of 1112 cases of melanoma.The Journal of molecular diagnostics : JMD · 2013Article
- Low-dose vemurafenib induces complete remission in a case of hairy-cell leukemia with a V600E mutation.Haematologica · 2013Article
- Simalikalactone E (SkE), a new weapon in the armamentarium of drugs targeting cancers that exhibit constitutive activation of the ERK pathway.Oncotarget · 2012Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
BRAF protooncogene is an important mediator of cell proliferation and survival signals. BRAF p.V600E mutation was recently described as a molecular marker of hairy cell leukemia (HCL). We developed and validated a pyrosequencing-based approach that covers BRAF mutational hotspots in exons 11 (codon 468) and 15 (codons 595 to 600). The assay detects BRAF mutations at an analytical sensitivity of 5%. We screened 16 unenriched archived bone marrow aspirate samples from patients with a diagnosis of HCL (n = 12) and hairy cell leukemia-variant (HCL-v) (n = 4) using pyrosequencing. BRAF p.V600E mutation was present in all HCL cases and absent in all HCL-v. Our data support the recent finding that BRAF p.V600E mutation is universally present in HCL. Moreover, our pyrosequencing-based assay provides a convenient, rapid, sensitive, and quantitative tool for the detection of BRAF p.V600E mutations in HCL for clinical diagnostic testing.
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