ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2012
Modulation of heat shock proteins during macrophage differentiation.
Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
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- Fullerene Derivatives for Tumor Treatment: Mechanisms and Application.International journal of nanomedicine · 2024Review
- Elevated human placental heat shock protein 5 is associated with spontaneous preterm birth.Pediatric research · 2023Article
- Hyperthermia promotes M1 polarization of macrophages via exosome-mediated HSPB8 transfer in triple negative breast cancer.Discover oncology · 2023Article
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- Macrophages in aseptic loosening: Characteristics, functions, and mechanisms.Frontiers in immunology · 2023Review
- Using Next-Generation Sequencing and Bioinformatic Methods to Predict New Genes That May Be Regulated by CD47 in Oral Squamous Cell Carcinoma.Current issues in molecular biology · 2022Article
- BrainCells · 2021Article
- Ductal Macrophages Predominate in the Immune Landscape of the Lactating Mammary Gland.Frontiers in immunology · 2021Article
- The role of tumor-associated macrophages (TAMs) in tumor progression and relevant advance in targeted therapy.Acta pharmaceutica Sinica. B · 2020Review
- Modulation of Tetraspanin 32 (TSPAN32) Expression in T Cell-Mediated Immune Responses and in Multiple Sclerosis.International journal of molecular sciences · 2019Article
- Exosomal Chaperones and miRNAs in Gliomagenesis: State-of-Art and Theranostics Perspectives.International journal of molecular sciences · 2018Review
- IL-4 up-regulates cyclooxygenase-1 expression in macrophages.The Journal of biological chemistry · 2017Article
- Population genetics analysis of the Nujiang catfish Creteuchiloglanis macropterus through a genome-wide single nucleotide polymorphisms resource generated by RAD-seq.Scientific reports · 2017Article
- Tumor-associated macrophages in cancers.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2016Review
- Evaluation of CHI3L-1 and CHIT-1 expression in differentiated and polarized macrophages.Inflammation · 2013Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo evaluate the heat shock protein (HSP) variation during the differentiation and polarization of human macrophages.
methodsGene expression analysis was investigated by real-time PCR from mRNA of human monocytes obtained from the buffy coats of healthy volunteers, polarized to classically activated macrophages (or M1), whose prototypical activating stimuli are interferon-gamma and lipopolysaccharide, and alternatively activated macrophages (or M2) obtained by interleukin-4 exposure. The modulation of HSPs at the transcriptomic levels was investigated using oligonucleotide microarray in the process of primary human monocyte-to-macrophage maturation and subsequent polarization into M1 or M2 cells.
resultsWe found that 11 HSPs transcripts were modulated throughout monocyte-to-macrophage differentiation. Furthermore a considerable effect on HSP expression was detected in conjunction with the M1 polarizing condition. This affected 21 transcripts in M1 cells, with 6 of them significantly upregulated in comparison to unpolarized macrophages, whereas 15 were downregulated. Slight changes in HSPs expression were observed in M2 cells when compared to unpolarized macrophages. Under these circumstances only five transcripts were significantly modulated. Interestingly, HSPBAP1 was the only HSP significantly downregulated in both M1 and M2 conditions parallel to a significant up-regulation of its target HSPB1.
conclusionOur study revealed that monocytes undergoing maturation differentially regulate the expression of several members of HSPs and that distinct patterns of HSP expression characterize the M1 and M2 effector stages of macrophage life.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.