ArticleThe Journal of clinical investigation2012
Intravital 2-photon imaging of leukocyte trafficking in beating heart.
Article in The Journal of clinical investigation, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
81 citing papers in PubMed, 119 citations in OpenAlex.
- Fate-mapping infiltrating monocytes following experimental myocardial infarction reveals differentiation trajectories in the infarcted heart.The Journal of clinical investigation · 2026Article
- NK cell allorecognition shapes reprogramming of neutrophils infiltrating heart allografts.bioRxiv : the preprint server for biology · 2025Article
- Tissue-resident CCR2Cell reports · 2025Article
- Editorial: Community series in intravital microscopy imaging of leukocytes, volume II.Frontiers in immunology · 2025Article
- Pericytes in mouse heart.Frontiers in physiology · 2025Article
- Hypoxia sensing in resident cardiac macrophages regulates monocyte fate specification following ischemic heart injury.Nature cardiovascular research · 2024Article
- B cells mediate lung ischemia/reperfusion injury by recruiting classical monocytes via synergistic B cell receptor/TLR4 signaling.The Journal of clinical investigation · 2024Article
- Intravital imaging of cardiac tissue utilizing tissue-stabilized heart window chamber in live animal model.European heart journal. Imaging methods and practice · 2024Article
- High dynamic range 3D motion tracking using circular scans with optical coherence tomography.Biomedical optics express · 2023Article
- Resolvin D1 prevents injurious neutrophil swarming in transplanted lungs.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A historical review of experimental imaging of the beating heart coronary microcirculation in vivo.Journal of anatomy · 2023Review
- Alpha4 beta7 integrin controls Th17 cell trafficking in the spinal cord leptomeninges during experimental autoimmune encephalomyelitis.Frontiers in immunology · 2023Article
- Nexinhib20 Inhibits Neutrophil Adhesion and βJournal of immunology (Baltimore, Md. : 1950) · 2022Article
- Nanoparticles reduce monocytes within the lungs to improve outcomes after influenza virus infection in aged mice.JCI insight · 2022Article
- Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs.Journal of visualized experiments : JoVE · 2022Article
- Immune cells in cardiac homeostasis and disease: emerging insights from novel technologies.European heart journal · 2022Review
- Necroptosis triggers spatially restricted neutrophil-mediated vascular damage during lung ischemia reperfusion injury.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Different Roles of Resident and Non-resident Macrophages in Cardiac Fibrosis.Frontiers in cardiovascular medicine · 2022Review
- Revealing the nanometric structural changes in myocardial infarction models by time-lapse intravital imaging.Frontiers in bioengineering and biotechnology · 2022Article
- GαJournal of immunology (Baltimore, Md. : 1950) · 2021Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Two-photon intravital microscopy has substantially broadened our understanding of tissue- and organ-specific differences in the regulation of inflammatory responses. However, little is known about the dynamic regulation of leukocyte recruitment into inflamed heart tissue, largely due to technical difficulties inherent in imaging moving tissue. Here, we report a method for imaging beating murine hearts using intravital 2-photon microscopy. Using this method, we visualized neutrophil trafficking at baseline and during inflammation. Ischemia reperfusion injury induced by transplantation or transient coronary artery ligation led to recruitment of neutrophils to the heart, their extravasation from coronary veins, and infiltration of the myocardium where they formed large clusters. Grafting hearts containing mutant ICAM-1, a ligand important for neutrophil recruitment, reduced the crawling velocities of neutrophils within vessels, and markedly inhibited their extravasation. Similar impairment was seen with the inhibition of Mac-1, a receptor for ICAM-1. Blockade of LFA-1, another ICAM-1 receptor, prevented neutrophil adherence to endothelium and extravasation in heart grafts. As inflammatory responses in the heart are of great relevance to public health, this imaging approach holds promise for studying cardiac-specific mechanisms of leukocyte recruitment and identifying novel therapeutic targets for treating heart disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.