ArticleThe Plant cell2012
The recent evolution of a symbiotic ion channel in the legume family altered ion conductance and improved functionality in calcium signaling.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 63 citations in OpenAlex.
- Putting channels in their place: nucleoporins and symbiotic signaling in legumes.Plant physiology · 2026Article
- The calcium signal regulatory network and the roles of calcium-binding proteins in bacteria.World journal of microbiology & biotechnology · 2026Review
- Autoactive MtDMI1 Reprogrammes Immunity and Development in Tomato via Ethylene Signalling.Plant biotechnology journal · 2026Article
- Review
- Constitutive activation of a nuclear-localized calcium channel complex inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Distinct Role of Mono-2-ethylhexyl Phthalate in Neuronal Transmission in Rat CA3 Hippocampal Neurons: Involvement of Ion Channels.Molecules (Basel, Switzerland) · 2022Article
- A Perspective on Developing a Plant 'Holobiont' for Future Saline Agriculture.Frontiers in microbiology · 2022Review
- Article
- Two plastid POLLUX ion channel-like proteins are required for stress-triggered stromal Ca2+release.Plant physiology · 2021Article
- Transport, functions, and interaction of calcium and manganese in plant organellar compartments.Plant physiology · 2021Review
- Genetics of nodulation in Aeschynomene evenia uncovers mechanisms of the rhizobium-legume symbiosis.Nature communications · 2021Article
- Nitrogen and Phosphorus Signaling and Transport During Legume-Rhizobium Symbiosis.Frontiers in plant science · 2021Review
- Nuclear calcium signatures are associated with root development.Nature communications · 2019Article
- The Ectomycorrhizal FungusThe Plant cell · 2019Article
- CaNature communications · 2019Article
- A set of Arabidopsis genes involved in the accommodation of the downy mildew pathogen Hyaloperonospora arabidopsidis.PLoS pathogens · 2019Article
- The Hydrophobin HYTLO1 Secreted by the Biocontrol FungusInternational journal of molecular sciences · 2018Article
- A Poly(A) Ribonuclease Controls the Cellotriose-Based Interaction betweenPlant physiology · 2018Article
- A Laser Dissection-RNAseq Analysis Highlights the Activation of Cytokinin Pathways by Nod Factors in the Medicago truncatula Root Epidermis.Plant physiology · 2016Article
- Does a Common Pathway Transduce Symbiotic Signals in Plant-Microbe Interactions?Frontiers in plant science · 2016Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arbuscular mycorrhiza and the rhizobia-legume symbiosis are two major root endosymbioses that facilitate plant nutrition. In Lotus japonicus, two symbiotic cation channels, CASTOR and POLLUX, are indispensable for the induction of nuclear calcium spiking, one of the earliest plant responses to symbiotic partner recognition. During recent evolution, a single amino acid substitution in DOES NOT MAKE INFECTIONS1 (DMI1), the POLLUX putative ortholog in the closely related Medicago truncatula, rendered the channel solo sufficient for symbiosis; castor, pollux, and castor pollux double mutants of L. japonicus were rescued by DMI1 alone, while both Lj-CASTOR and Lj-POLLUX were required for rescuing a dmi1 mutant of M. truncatula. Experimental replacement of the critical serine by an alanine in the selectivity filter of Lj-POLLUX conferred a symbiotic performance indistinguishable from DMI1. Electrophysiological characterization of DMI1 and Lj-CASTOR (wild-type and mutants) by planar lipid bilayer experiments combined with calcium imaging in Human Embryonic Kidney-293 cells expressing DMI1 (the wild type and mutants) suggest that the serine-to-alanine substitution conferred reduced conductance with a long open state to DMI1 and improved its efficiency in mediating calcium oscillations. We propose that this single amino acid replacement in the selectivity filter made DMI1 solo sufficient for symbiosis, thus explaining the selective advantage of this allele at the mechanistic level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.