ArticleApplied and environmental microbiology2012
Expression of the laccase gene from a white rot fungus in Pichia pastoris can enhance the resistance of this yeast to H2O2-mediated oxidative stress by stimulating the glutathione-based antioxidative system.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Decoding the distribution, structure-function-redox potential relationship and recent advances in fungal laccases: a systematic approach.Bioprocess and biosystems engineering · 2026Review
- RcAlb-PepII Perturbs the Proteomic Profile ofMicroorganisms · 2026Article
- The regulatory network ofMicrobiology spectrum · 2025Article
- Astral-based DIA proteomics analysis reveals the effects of selenium on Pleurotus pulmonarius.Scientific reports · 2025Article
- Transcriptom Analysis ofMycobiology · 2025Article
- Transcriptomic and metabolomic analysis unveils a negative effect of glutathione metabolism on laccase activity inMicrobiology spectrum · 2024Article
- Cinnamaldehyde Inhibits Postharvest Gray Mold on Pepper Fruits via Inhibiting Fungal Growth and Triggering Fruit Defense.Foods (Basel, Switzerland) · 2023Article
- Deciphering the Effect of Light Wavelengths inJournal of fungi (Basel, Switzerland) · 2023Article
- Enhancing the Biocontrol Potential of the Entomopathogenic Fungus in Multiple Respects via the Overexpression of a Transcription Factor GeneJournal of fungi (Basel, Switzerland) · 2022Article
- Coprinopsis cinerea Uses Laccase Lcc9 as a Defense Strategy To Eliminate Oxidative Stress during Fungal-Fungal Interactions.Applied and environmental microbiology · 2022Article
- Antioxidant effect of yeast on lipid oxidation in salami sausage.Frontiers in microbiology · 2022Article
- Evidence for Lignocellulose-Decomposing Enzymes in the Genome and Transcriptome of the Aquatic HyphomyceteJournal of fungi (Basel, Switzerland) · 2021Article
- Effect of tebuconazole and trifloxystrobin on Ceratocystis fimbriata to control black rot of sweet potato: processes of reactive oxygen species generation and antioxidant defense responses.World journal of microbiology & biotechnology · 2021Article
- Genome-based engineering of ligninolytic enzymes in fungi.Microbial cell factories · 2021Review
- A Cytochrome BFrontiers in plant science · 2021Article
- The Antioxidant Guaiacol Exerts Fungicidal Activity Against Fungal Growth and Deoxynivalenol Production inFrontiers in microbiology · 2021Article
- Comparative RNAseq Analysis of the Insect-Pathogenic FungusG3 (Bethesda, Md.) · 2020Article
- Transcriptome analysis of differential gene expression in Dichomitus squalens during interspecific mycelial interactions and the potential link with laccase induction.Journal of microbiology (Seoul, Korea) · 2019Article
- Metal release and sequestration from black slate mediated by a laccase of Schizophyllum commune.Environmental science and pollution research international · 2019Article
- Dietary magnesium deficiency impaired intestinal structural integrity in grass carp (Ctenopharyngodon idella).Scientific reports · 2018Article
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8 authors.
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Abstract
Laccase is a copper-containing polyphenol oxidase that has great potential in industrial and biotechnological applications. Previous research has suggested that fungal laccase may be involved in the defense against oxidative stress, but there is little direct evidence supporting this hypothesis, and the mechanism by which laccase protects cells from oxidative stress also remains unclear. Here, we report that the expression of the laccase gene from white rot fungus in Pichia pastoris can significantly enhance the resistance of yeast to H(2)O(2)-mediated oxidative stress. The expression of laccase in yeast was found to confer a strong ability to scavenge intracellular H(2)O(2) and to protect cells from lipid oxidative damage. The mechanism by which laccase gene expression increases resistance to oxidative stress was then investigated further. We found that laccase gene expression in Pichia pastoris could increase the level of glutathione-based antioxidative activity, including the intracellular glutathione levels and the enzymatic activity of glutathione peroxidase, glutathione reductase, and γ-glutamylcysteine synthetase. The transcription of the laccase gene in Pichia pastoris was found to be enhanced by the oxidative stress caused by exogenous H(2)O(2). The stimulation of laccase gene expression in response to exogenous H(2)O(2) stress further contributed to the transcriptional induction of the genes involved in the glutathione-dependent antioxidative system, including PpYAP1, PpGPX1, PpPMP20, PpGLR1, and PpGSH1. Taken together, these results suggest that the expression of the laccase gene in Pichia pastoris can enhance the resistance of yeast to H(2)O(2)-mediated oxidative stress by stimulating the glutathione-based antioxidative system to protect the cell from oxidative damage.
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