ArticleBioengineered
PanDaTox: a tool for accelerated metabolic engineering.
Article in Bioengineered. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Bioengineering tools for the production of pharmaceuticals: current perspective and future outlook.Bioengineered · 2019Review
- Reconstruction of real and simulated phylogenies based on quartet plurality inference.BMC genomics · 2018Article
- Phylogenomic networks reveal limited phylogenetic range of lateral gene transfer by transduction.The ISME journal · 2017Article
- Codon reassignment to facilitate genetic engineering and biocontainment in the chloroplast of Chlamydomonas reinhardtii.Plant biotechnology journal · 2016Article
- A set of powerful negative selection systems for unmodified Enterobacteriaceae.Nucleic acids research · 2015Article
- Computational evaluation of cellular metabolic costs successfully predicts genes whose expression is deleterious.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
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2 authors.
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Abstract
Metabolic engineering is often facilitated by cloning of genes encoding enzymes from various heterologous organisms into E. coli. Such engineering efforts are frequently hampered by foreign genes that are toxic to the E. coli host. We have developed PanDaTox (www.weizmann.ac.il/pandatox), a web-based resource that provides experimental toxicity information for more than 1.5 million genes from hundreds of different microbial genomes. The toxicity predictions, which were extensively experimentally verified, are based on serial cloning of genes into E. coli as part of the Sanger whole genome shotgun sequencing process. PanDaTox can accelerate metabolic engineering projects by allowing researchers to exclude toxic genes from the engineering plan and verify the clonability of selected genes before the actual metabolic engineering experiments are conducted.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.