ArticleActa biomaterialia2012
Human corneal limbal epithelial cell response to varying silk film geometric topography in vitro.
Article in Acta biomaterialia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Corneal Epithelial Tissue Engineering Strategy Based on Cell Viability Optimization: A Review and Prospects.Bioengineering (Basel, Switzerland) · 2025Review
- Silk Fibroin Surface Engineering Using Phase Separation Approaches for Enhanced Cell Adhesion and Proliferation.ACS applied materials & interfaces · 2025Article
- Substrate engineering using naturally biomimicking corneal cell topography for preserving stemness of corneal limbal epithelial-stem cells.Iranian journal of basic medical sciences · 2025Article
- Insight and Recent Advances into the Role of Topography on the Cell Differentiation and Proliferation on Biopolymeric Surfaces.International journal of molecular sciences · 2022Review
- Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury.Experimental eye research · 2022Article
- Silk films with nanotopography and extracellular proteins enhance corneal epithelial wound healing.Scientific reports · 2021Article
- Modeling the cornea in 3-dimensions: Current and future perspectives.Experimental eye research · 2020Review
- Plasticised Regenerated Silk/Gold Nanorods Hybrids as Sealant and Bio-Piezoelectric Materials.Nanomaterials (Basel, Switzerland) · 2020Article
- Bionic Silk Fibroin Film Induces Morphological Changes and Differentiation of Tendon Stem/Progenitor Cells.Applied bionics and biomechanics · 2020Article
- Engineering topography: Effects on corneal cell behavior and integration into corneal tissue engineering.Bioactive materials · 2019Review
- Silk Hydrogels Crosslinked by the Fenton Reaction.Advanced healthcare materials · 2019Article
- The Effect of Micro- and Nanoscale Surface Topographies on Silk on Human Corneal Limbal Epithelial Cell Differentiation.Scientific reports · 2019Article
- A Review of the Emerging Role of Silk for the Treatment of the Eye.Pharmaceutical research · 2018Review
- Combining Living Microorganisms with Regenerated Silk Provides Nanofibril-Based Thin Films with Heat-Responsive Wrinkled States for Smart Food Packaging.Nanomaterials (Basel, Switzerland) · 2018Article
- Multi-layered silk film coculture system for human corneal epithelial and stromal stem cells.Journal of tissue engineering and regenerative medicine · 2018Article
- Micro- and Nanoscale Topographies on Silk Regulate Gene Expression of Human Corneal Epithelial Cells.Investigative ophthalmology & visual science · 2017Article
- [New biomaterials and alternative stem cell sources for the reconstruction of the limbal stem cell niche].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2017Review
- 3D Functional Corneal Stromal Tissue Equivalent Based on Corneal Stromal Stem Cells and Multi-Layered Silk Film Architecture.PloS one · 2017Article
- Silk Fibroin as Edible Coating for Perishable Food Preservation.Scientific reports · 2016Article
- Optimization of Corneal Epithelial Progenitor Cell Growth on Bombyx mori Silk Fibroin Membranes.Stem cells international · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Silk fibroin films are a promising class of biomaterials that have a number of advantages for use in ophthalmic applications due to their transparent nature, mechanical properties and minimal inflammatory response upon implantation. Freestanding silk films with parallel line and concentric ring topographies were generated for in vitro characterization of human corneal limbal epithelial (HCLE) cell response upon differing geometric patterned surfaces. Results indicated that silk film topography significantly affected initial HCLE culture substrate attachment, cellular alignment, cell-to-cell contact formation, actin cytoskeleton alignment and focal adhesion (FA) localization. Most notably, parallel line patterned surfaces displayed a 36-54% increase on average in initial cell attachment, which corresponded to a more than 2-fold increase in FA localization when compared to other silk film surfaces and controls. In addition, distinct localization of FA formation was observed along the edges for all patterned silk film topographies. In conclusion, silk film feature topography appears to help direct corneal epithelial cell response and cytoskeleton development, especially with regard to FA distribution, in vitro.
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Registered trials
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