ArticleCurrent biology : CB2012
Tropomyosin is essential for processive movement of a class V myosin from budding yeast.
Article in Current biology : CB, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 64 citations in OpenAlex.
- Functional redundancy and formin-isoform independent localization of tropomyosin paralogs in Saccharomyces cerevisiae.PLoS genetics · 2025Article
- The actin module of endocytic internalization in Aspergillus nidulans: A critical role of the WISH/DIP/SPIN90 family protein Dip1.PLoS genetics · 2025Article
- Myosin-1C differentially displaces tropomyosin isoforms altering their inhibition of motility.The Journal of biological chemistry · 2024Article
- Nonmuscle myosin 2 filaments are processive in cells.Biophysical journal · 2023Article
- Single-molecule mechanics and kinetics of cardiac myosin interacting with regulated thin filaments.Biophysical journal · 2023Article
- Visualizing the in vitro assembly of tropomyosin/actin filaments using TIRF microscopy.Biophysical reviews · 2020Review
- Distinct sites in tropomyosin specify shared and isoform-specific regulation of myosins II and V.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Three mammalian tropomyosin isoforms have different regulatory effects on nonmuscle myosin-2B and filamentous β-actinThe Journal of biological chemistry · 2018Article
- Kinetic signatures of myosin-5B, the motor involved in microvillus inclusion disease.The Journal of biological chemistry · 2017Article
- Fission yeast myosin Myo2 is down-regulated in actin affinity by light chain phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Tropomyosin Isoforms Specify Functionally Distinct Actin Filament Populations In Vitro.Current biology : CB · 2017Article
- Tropomyosin isoforms bias actin track selection by vertebrate myosin Va.Molecular biology of the cell · 2016Article
- Analysis of biophysical and functional consequences of tropomyosin-fluorescent protein fusions.FEBS letters · 2016Article
- Prion Aggregates Are Recruited to the Insoluble Protein Deposit (IPOD) via Myosin 2-Based Vesicular Transport.PLoS genetics · 2016Article
- Myosin Vc Is Specialized for Transport on a Secretory Superhighway.Current biology : CB · 2016Article
- Various Themes of Myosin Regulation.Journal of molecular biology · 2016Review
- Load-dependent modulation of non-muscle myosin-2A function by tropomyosin 4.2.Scientific reports · 2016Article
- Computational model of polarized actin cables and cytokinetic actin ring formation in budding yeast.Cytoskeleton (Hoboken, N.J.) · 2015Article
- Actin age orchestrates myosin-5 and myosin-6 run lengths.Current biology : CB · 2015Article
- Evolutionarily conserved sites in yeast tropomyosin function in cell polarity, transport and contractile ring formation.Biology open · 2015Article
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6 authors at 1 institution in 1 country.
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Abstract
Myosin V is an actin-based motor protein involved in intracellular cargo transport [1]. Given this physiological role, it was widely assumed that all class V myosins are processive, able to take multiple steps along actin filaments without dissociating. This notion was challenged when several class V myosins were characterized as nonprocessive in vitro, including Myo2p, the essential class V myosin from S. cerevisiae [2-6]. Myo2p moves cargo including secretory vesicles and other organelles for several microns along actin cables in vivo. This demonstrated cargo transporter must therefore either operate in small ensembles or behave processively in the cellular context. Here we show that Myo2p moves processively in vitro as a single motor when it walks on an actin track that more closely resembles the actin cables found in vivo. The key to processivity is tropomyosin: Myo2p is not processive on bare actin but highly processive on actin-tropomyosin. The major yeast tropomyosin isoform, Tpm1p, supports the most robust processivity. Tropomyosin slows the rate of MgADP release, thus increasing the time the motor spends strongly attached to actin. This is the first example of tropomyosin switching a motor from nonprocessive to processive motion on actin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.