ArticleMicrobial cell factories2012
Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Metabolic engineering ofApplied and environmental microbiology · 2026Review
- Integrated Fermentation Engineering Enables High-Level Leghemoglobin Production in Kluyveromyces marxianus via Metabolic Rewiring of Central Carbon Metabolism and Amino Acid Utilization.Biotechnology journal · 2026Article
- Expression of functional human sialyltransferases ST6GalNAc5 and ST6GalNAc6 in Pichia pastoris.Applied microbiology and biotechnology · 2025Article
- Impact of Oxygen Availability on the Organelle-Specific Redox Potentials and Stress in Recombinant Protein Producing Komagataella phaffii.Microbial biotechnology · 2025Article
- Large scale microfluidic CRISPR screening for increased amylase secretion in yeast.Lab on a chip · 2023Article
- Enhanced Recombinant Protein Production Under Special Environmental Stress.Frontiers in microbiology · 2021Review
- Rationale-based selection of optimal operating strategies and gene dosage impact on recombinant protein production in Komagataella phaffii (Pichia pastoris).Microbial biotechnology · 2020Article
- Continuous Cultivation as a Tool Toward the Rational Bioprocess Development WithFrontiers in bioengineering and biotechnology · 2020Review
- Harnessing microbial metabolomics for industrial applications.World journal of microbiology & biotechnology · 2019Review
- Alteration of Amino Acid Profiling Influenced by the Active Ingredients of DanHong Injection After Prescription Optimization.Drug design, development and therapy · 2019Article
- Process development for the continuous production of heterologous proteins by the industrial yeast, Komagataella phaffii.Biotechnology and bioengineering · 2018Article
- The effect of hypoxia on the lipidome of recombinant Pichia pastoris.Microbial cell factories · 2017Article
- Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling.Journal of industrial microbiology & biotechnology · 2017Article
- Comprehensive reconstruction and evaluation ofBioresources and bioprocessing · 2017Article
- Article
- Integrated isotope-assisted metabolomics and (13)C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger.Microbial cell factories · 2015Article
- Engineering the supply chain for protein production/secretion in yeasts and mammalian cells.Journal of industrial microbiology & biotechnology · 2015Review
- Improving functional annotation for industrial microbes: a case study with Pichia pastoris.Trends in biotechnology · 2014Article
- Article
- Yeast biotechnology: teaching the old dog new tricks.Microbial cell factories · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnvironmental and intrinsic stress factors can result in the global alteration of yeast physiology, as evidenced by several transcriptional studies. Hypoxia has been shown to have a beneficial effect on the expression of recombinant proteins in Pichia pastoris growing on glucose. Furthermore, transcriptional profiling analyses revealed that oxygen availability was strongly affecting ergosterol biosynthesis, central carbon metabolism and stress responses, in particular the unfolded protein response. To contribute to the better understanding of the effect and interplay of oxygen availability and foreign protein secretion on central metabolism, a first quantitative metabolomic analysis of free amino acids pools in a recombinant P. pastoris strain growing under different oxygen availability conditions has been performed.
resultsThe values obtained indicate significant variations in the intracellular amino acid pools due to different oxygen availability conditions, showing an overall increase of their size under oxygen limitation. Notably, even while foreign protein productivities were relatively low (about 40-80 μg Fab/g(DCW)·h), recombinant protein production was found to have a limited but significant impact on the intracellular amino acid pools, which were generally decreased in the producing strain compared with the reference strain. However, observed changes in individual amino acids pools were not correlated with their corresponding relative abundance in the recombinant protein sequence, but to the overall cell protein amino acid compositional variations.
conclusionsOverall, the results obtained, combined with previous transcriptomic and proteomic analyses provide a systematic metabolic fingerprint of the oxygen availability impact on recombinant protein production in P. pastoris.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.