ArticleBMC cancer2012
Characterization of human gastric carcinoma-related methylation of 9 miR CpG islands and repression of their expressions in vitro and in vivo.
Article in BMC cancer, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 39 citations in OpenAlex.
- miR-2682-3p antagonizes its host lncRNA-MIR137HG by interacting with the same target FUS to regulate the progression of gastric cancer.BMC cancer · 2022Article
- MicroRNA-193b impairs muscle growth in mouse models of type 2 diabetes by targeting the PDK1/Akt signalling pathway.Diabetologia · 2022Article
- Methylation-associated miR-193b silencing activates master drivers of aggressive prostate cancer.Molecular oncology · 2019Article
- Sulforaphane epigenetically demethylates the CpG sites of the miR-9-3 promoter and reactivates miR-9-3 expression in human lung cancer A549 cells.The Journal of nutritional biochemistry · 2018Article
- MicroRNA-203 mimics age-related aortic smooth muscle dysfunction of cytoskeletal pathways.Journal of cellular and molecular medicine · 2017Article
- miR-137 plays tumor suppressor roles in gastric cancer cell lines by targeting KLF12 and MYO1C.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Aberrant methylation-mediated silencing of microRNAs contributes to HPV-induced anchorage independence.Oncotarget · 2016Article
- Detection of ALDH1 activity in rabbit hepatic VX2 tumors and isolation of ALDH1 positive cancer stem cells.Journal of translational medicine · 2016Article
- Dysregulation of non-coding RNAs in gastric cancer.World journal of gastroenterology · 2015Review
- MicroRNA library-based functional screening identified miR-137 as a suppresser of gastric cancer cell proliferation.Journal of cancer research and clinical oncology · 2015Article
- DNA methylation, microRNAs, and their crosstalk as potential biomarkers in hepatocellular carcinoma.World journal of gastroenterology · 2014Review
- Survival of motor neuron protein downregulates miR-9 expression in patients with spinal muscular atrophy.The Kaohsiung journal of medical sciences · 2014Article
- Hypoxia-inducible factor 1-α induces miR-210 in normoxic differentiating myoblasts.The Journal of biological chemistry · 2012Article
- MicroRNAs in cancers and neurodegenerative disorders.Frontiers in genetics · 2012Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMany miR genes are located within or around CpG islands. It is unclear whether methylation of these CpG islands represses miR transcription regularly. The aims of this study are to characterize gastric carcinoma (GC)-related methylation of miR CpG islands and its relationship with miRNA expression.
methodsMethylation status of 9 representative miR CpG islands in a panel of cell lines and human gastric samples (including 13 normal biopsies, 38 gastritis biopsies, 112 pairs of GCs and their surgical margin samples) was analyzed by bisulfite-DHPLC and sequencing. Mature miRNA levels were determined with quantitative RT-PCR. Relationships between miR methylation, transcription, GC development, and clinicopathological characteristics were statistically analyzed.
resultsMethylation frequency of 5 miR CpG islands (miR-9-1, miR-9-3, miR-137, miR-34b, and miR-210) gradually increased while the proportion of methylated miR-200b gradually decreased during gastric carcinogenesis (Ps < 0.01). More miR-9-1 methylation was detected in 62%-64% of the GC samples and 4% of the normal or gastritis samples (18/28 versus 2/48; Odds ratio, 41.4; P < 0.01). miR-210 methylation showed high correlation with H. pylori infection. miR-375, miR-203, and miR-193b methylation might be host adaptation to the development of GCs. Methylation of these miR CpG islands was consistently shown to significantly decrease the corresponding miRNA levels presented in human cell lines. The inverse relationship was also observed for miR-9-1, miR-9-3, miR-137, and miR-200b in gastric samples. Among 112 GC patients, miR-9-1 methylation was an independent favourable predictor of overall survival of GC patients in both univariate and multivariate analysis (P < 0.02).
conclusionsIn conclusion, alteration of methylation status of 6 of 9 tested miR CpG islands was characterized in gastric carcinogenesis. miR-210 methylation correlated with H. pylori infection. miR-9-1 methylation may be a GC-specific event. Methylation of miR CpG islands may significantly down-regulate their transcription regularly.
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