ArticleTissue engineering. Part A2012
Microtissue engineered constructs with living axons for targeted nervous system reconstruction.
Article in Tissue engineering. Part A, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 73 citations in OpenAlex.
- Optogenetic-enabled biomanufacturing of long-projecting motor tracts for neural reconstruction.Biofabrication · 2026Article
- Transforming healthcare through in-body bioelectronic systems.Nature communications · 2026Review
- Bio-inspired electronics: Soft, biohybrid, and "living" neural interfaces.Nature communications · 2025Review
- The Future of Biohybrid Regenerative Bioelectronics.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Biomaterial strategies for regulating the neuroinflammatory response.Materials advances · 2024Review
- Injectable Hydrogels for Nervous Tissue Repair-A Brief Review.Gels (Basel, Switzerland) · 2024Review
- Generation of contractile forces by three-dimensional bundled axonal tracts in micro-tissue engineered neural networks.Frontiers in molecular neuroscience · 2024Article
- Editorial: Perspectives in neuroscience: mechanical forces for the modulation of axonal mechanics and nerve regeneration.Frontiers in molecular neuroscience · 2024Article
- Cell Replacement Therapy for Brain Repair: Recent Progress and Remaining Challenges for Treating Parkinson's Disease and Cortical Injury.Brain sciences · 2023Review
- Engineered neuronal microtissue provides exogenous axons for delayed nerve fusion and rapid neuromuscular recovery in rats.Bioactive materials · 2022Article
- Axonal Tract Reconstruction Using a Tissue-Engineered Nigrostriatal Pathway in a Rat Model of Parkinson's Disease.International journal of molecular sciences · 2022Article
- New Targets and New Technologies in the Treatment of Parkinson's Disease: A Narrative Review.International journal of environmental research and public health · 2022Review
- Intra-articular MMP-1 in the spinal facet joint induces sustained pain and neuronal dysregulation in the DRG and spinal cord, and alters ligament kinematics under tensile loading.Frontiers in bioengineering and biotechnology · 2022Article
- Local tissue heterogeneity may modulate neuronal responses via altered axon strain fields: insights about innervated joint capsules from a computational model.Biomechanics and modeling in mechanobiology · 2021Article
- Implantation of Engineered Axon Tracts to Bridge Spinal Cord Injury Beyond the Glial Scar in Rats.Tissue engineering. Part A · 2021Article
- Restoring lost nigrostriatal fibers in Parkinson's disease based on clinically-inspired design criteria.Brain research bulletin · 2021Review
- Development and maintenance of vision's first synapse.Developmental biology · 2021Review
- An implantable human stem cell-derived tissue-engineered rostral migratory stream for directed neuronal replacement.Communications biology · 2021Article
- Intra-articular collagenase in the spinal facet joint induces pain, DRG neuron dysregulation and increased MMP-1 absent evidence of joint destruction.Scientific reports · 2020Article
- Emerging regenerative medicine and tissue engineering strategies for Parkinson's disease.NPJ Parkinson's disease · 2020Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
As a common feature of many neurological diseases and injury, the loss of axon pathways can have devastating effects on function. Here, we demonstrate a new strategy to restore damaged axon pathways using transplantable miniature constructs consisting of living neurons and axonal tracts internalized within hydrogel tubes. These hydrogel microconduits were developed through an iterative process to support neuronal survival and directed axon growth. The design included hollow agarose tubes providing a relatively stiff outer casing to direct constrained unidirectional outgrowth of axons through a central soft collagen matrix, with overall dimensions of 250 μm inner diameter ×500 μm outer diameter and extending up to several centimeters. The outer casing was also designed to provide structural support of neuronal/axonal cultures during transplantation of the construct. Using neuron culture conditions optimized for the microconduits, dissociated dorsal root ganglia neurons were seeded in the collagen at one end of the conduits. Over the following week, high-resolution confocal microscopy demonstrated that the neurons survived and the somata remained in a tight cluster at the original seeding site. In addition, robust outgrowth of axons from the neurons was found, with axon fascicles constrained in a longitudinal projection along the internal collagen canal and extending over 5 mm in length. Notably, this general geometry recapitulates the anatomy of axon tracts. As such, these constructs may be useful to repair damaged axon projections by providing a transplantable bridge of living axons. Moreover, the small size of the construct permits follow-on studies of minimally invasive transplantation into potentially sensitive regions of the nervous system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.