ArticlePloS one2012
The Neurospora crassa dfg5 and dcw1 genes encode α-1,6-mannanases that function in the incorporation of glycoproteins into the cell wall.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Characterization of the Polysaccharide and Protein Composition ofJournal of fungi (Basel, Switzerland) · 2026Article
- Biochemical characterization of the α-1,3-mannosidase AnGH92A from Aspergillus nidulans.Scientific reports · 2026Article
- Article
- Characterization of theCell surface (Amsterdam, Netherlands) · 2025Article
- Article
- Characterization of theMicroorganisms · 2024Article
- Structural diversification of fungal cell wall in response to the stress signaling and remodeling during fungal pathogenesis.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- The cell functions of phospholipase C-1, CaArchives of microbiology · 2023Article
- Extracellular targeting of Neurospora crassa cell wall and secreted glycoproteins by DFG-5.Fungal genetics and biology : FG & B · 2022Article
- The GUL-1 Protein Binds Multiple RNAs Involved in Cell Wall Remodeling and Affects the MAK-1 Pathway inFrontiers in fungal biology · 2021Article
- The GPI-Anchored GH76 Protein Dfg5 Affects Hyphal Morphology and Osmoregulation in the MycoparasiteFrontiers in microbiology · 2021Article
- Evolutionary Overview of Molecular Interactions and Enzymatic Activities in the Yeast Cell Walls.International journal of molecular sciences · 2020Review
- Galactomannan Produced byJournal of fungi (Basel, Switzerland) · 2020Review
- Structural base for the transfer of GPI-anchored glycoproteins into fungal cell walls.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris.BMC microbiology · 2020Article
- Off the wall: The rhyme and reason ofCell surface (Amsterdam, Netherlands) · 2019Review
- The Glycosylphosphatidylinositol-AnchoredmSphere · 2019Article
- Mechanisms of redundancy and specificity of the Aspergillus fumigatus Crh transglycosylases.Nature communications · 2019Article
- The Genetics and Biochemistry of Cell Wall Structure and Synthesis inFrontiers in microbiology · 2019Review
- The Farnesyltransferase β-Subunit Ram1 RegulatesFrontiers in microbiology · 2019Article
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3 authors.
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Abstract
The covalent cross-linking of cell wall proteins into the cell wall glucan/chitin matrix is an important step in the biogenesis of the fungal cell wall. We demonstrate that the Neurospora crassa DFG5 (NCU03770) and DCW1 (NCU08127) enzymes function in vivo to cross-link glycoproteins into the cell wall. Mutants lacking DFG5 or DCW1 release slightly elevated levels of cell wall proteins into their growth medium. Mutants lacking both DFG5 and DCW1 have substantially reduced levels of cell wall proteins in their cell walls and release large amounts of known cell wall proteins into the medium. DFG5 and DCW1 are members of the GH76 family of glycosyl hydrolases, which have specificity to recognize and cleave α-1,6-mannans. A model for incorporation of glycoproteins into the cell wall through the α-1,6-mannan core of the N-linked galactomannan is presented. In this model, DFG5 and DCW1 recognize the N-linked galactomannan present on glycoproteins and cross-link it into the cell wall glucan/chitin matrix.
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