ArticlePloS one2012
Intracellular vesicles as reproduction elements in cell wall-deficient L-form bacteria.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Lactic acid bacteria and their bacteriocins: promising food safety tools for controlling the foodborne pathogen Listeria monocytogenes.World journal of microbiology & biotechnology · 2026Review
- The Flavonoids Daidzein and Genistein Induce Wall-Deficient Cell Formation in Streptomyces coelicolor Under Hyperosmotic Stress.Microbial biotechnology · 2026Article
- Synergistic action of peptidoglycan and teichoic acid synthesis inhibitors leads to cell death by oxidative damage.Communications biology · 2026Article
- Revealing Cell Envelope Heterogeneity in Two StableInternational journal of molecular sciences · 2026Article
- Essential and dispensable domains of DivIVA for walled growth in filamentous Actinomycetota.Open biology · 2025Article
- Peptidoglycan-reshuffling proteins SCO0954, SCO1758, SCO4439, and SCO4440 modulate the formation of wall-deficient cells in Streptomyces coelicolor under hyperosmotic sucrose stress.Scientific reports · 2025Article
- The reproduction of gram-negative protoplasts and the influence of environmental conditions on this process.iScience · 2023Article
- Comparative genomics hints at dispensability of multiple essential genes in two Escherichia coli L-form strains.Bioscience reports · 2023Article
- L-form conversion in Gram-positive bacteria enables escape from phage infection.Nature microbiology · 2023Article
- Endocytosis-like DNA uptake by cell wall-deficient bacteria.Nature communications · 2022Article
- Cross-Genus "Boot-Up" of Synthetic Bacteriophage in Staphylococcus aureus by Using a New and Efficient DNA Transformation Method.Applied and environmental microbiology · 2022Article
- Ampicillin Treatment of IntracellularFrontiers in cellular and infection microbiology · 2022Article
- Species-dependent protoplast enlargement involves different types of vacuole generation in bacteria.Scientific reports · 2020Article
- The Concept of an Ideal Antibiotic: Implications for Drug Design.Molecules (Basel, Switzerland) · 2019Review
- Proliferation of Listeria monocytogenes L-form cells by formation of internal and external vesicles.Nature communications · 2016Article
- The Absence of a Mature Cell Wall Sacculus in Stable Listeria monocytogenes L-Form Cells Is Independent of Peptidoglycan Synthesis.PloS one · 2016Article
- Morphological and biochemical features of Borrelia burgdorferi pleomorphic forms.Microbiology (Reading, England) · 2015Article
- Molecular complementarity between simple, universal molecules and ions limited phenotype space in the precursors of cells.Biology direct · 2014Article
- Divided we stand: splitting synthetic cells for their proliferation.Systems and synthetic biology · 2014Review
- New approaches to the problem of generating coherent, reproducible phenotypes.Theory in biosciences = Theorie in den Biowissenschaften · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell wall-deficient bacteria, or L-forms, represent an extreme example of bacterial plasticity. Stable L-forms can multiply and propagate indefinitely in the absence of a cell wall. Data presented here are consistent with the model that intracellular vesicles in Listeria monocytogenes L-form cells represent the actual viable reproductive elements. First, small intracellular vesicles are formed along the mother cell cytoplasmic membrane, originating from local phospholipid accumulation. During growth, daughter vesicles incorporate a small volume of the cellular cytoplasm, and accumulate within volume-expanding mother cells. Confocal Raman microspectroscopy demonstrated the presence of nucleic acids and proteins in all intracellular vesicles, but only a fraction of which reveals metabolic activity. Following collapse of the mother cell and release of the daughter vesicles, they can establish their own membrane potential required for respiratory and metabolic processes. Premature depolarization of the surrounding membrane promotes activation of daughter cell metabolism prior to release. Based on genome resequencing of L-forms and comparison to the parental strain, we found no evidence for predisposing mutations that might be required for L-form transition. Further investigations revealed that propagation by intracellular budding not only occurs in Listeria species, but also in L-form cells generated from different Enterococcus species. From a more general viewpoint, this type of multiplication mechanism seems reminiscent of the physicochemical self-reproducing properties of abiotic lipid vesicles used to study the primordial reproduction pathways of putative prokaryotic precursor cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.