ArticlePloS one2012
Mechanobiological modulation of cytoskeleton and calcium influx in osteoblastic cells by short-term focused acoustic radiation force.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed.
- Flexible Dielectric Acoustic Resonator Patch for Tissue Regeneration.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Application of Focused Ultrasound for the Treatment of Tendinopathy: An In Vivo Feasibility, Safety, and Efficacy Study.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- LIPUS regulates the progression of knee osteoarthritis in mice through primary cilia-mediated TRPV4 channels.Apoptosis : an international journal on programmed cell death · 2024Article
- Vascular Smooth Muscle Cells Transdifferentiate into Chondrocyte-Like Cells and Facilitate Meniscal Fibrocartilage Regeneration.Research (Washington, D.C.) · 2024Article
- The release of osteoclast-stimulating factors on supraphysiological loading by osteoprogenitors coincides with expression of genes associated with inflammation and cytoskeletal arrangement.Scientific reports · 2022Article
- Development of a Novel Perfusion Rotating Wall Vessel Bioreactor with Ultrasound Stimulation for Mass-Production of Mineralized Tissue Constructs.Tissue engineering and regenerative medicine · 2022Article
- Acoustofluidic Stimulation of Functional Immune Cells in a Microreactor.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Focused ultrasound excites cortical neurons via mechanosensitive calcium accumulation and ion channel amplification.Nature communications · 2022Article
- Piezo1 channel activation in response to mechanobiological acoustic radiation force in osteoblastic cells.Bone research · 2021Article
- The waves that make the pattern: a review on acoustic manipulation in biomedical research.Materials today. Bio · 2021Review
- Mechanisms and Applications of Neuromodulation Using Surface Acoustic Waves-A Mini-Review.Frontiers in neuroscience · 2021Review
- Acoustofluidic phase microscopy in a tilted segmentation-free configuration.Biomicrofluidics · 2021Article
- Sounds Stimulation on In Vitro HL1 Cells: A Pilot Study and a Theoretical Physical Model.International journal of molecular sciences · 2020Article
- Low-dose X-ray irradiation induces morphological changes and cytoskeleton reorganization in osteoblasts.Experimental and therapeutic medicine · 2020Article
- Acoustofluidic methods in cell analysis.Trends in analytical chemistry : TRAC · 2019Article
- Frequency-induced morphology alterations in microconfined biological cells.Medical & biological engineering & computing · 2019Article
- Mitigation of Articular Cartilage Degeneration and Subchondral Bone Sclerosis in Osteoarthritis Progression Using Low-Intensity Ultrasound Stimulation.Ultrasound in medicine & biology · 2019Article
- Production of acoustic radiation force using ultrasound: methods and applications.Expert review of medical devices · 2018Review
- Sonoporation-induced cell membrane permeabilization and cytoskeleton disassembly at varied acoustic and microbubble-cell parameters.Scientific reports · 2018Article
- Theoretically proposed optimal frequency for ultrasound induced cartilage restoration.Theoretical biology & medical modelling · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Mechanotransduction has demonstrated potential for regulating tissue adaptation in vivo and cellular activities in vitro. It is well documented that ultrasound can produce a wide variety of biological effects in biological systems. For example, pulsed ultrasound can be used to noninvasively accelerate the rate of bone fracture healing. Although a wide range of studies has been performed, mechanism for this therapeutic effect on bone healing is currently unknown. To elucidate the mechanism of cellular response to mechanical stimuli induced by pulsed ultrasound radiation, we developed a method to apply focused acoustic radiation force (ARF) (duration, one minute) on osteoblastic MC3T3-E1 cells and observed cellular responses to ARF using a spinning disk confocal microscope. This study demonstrates that the focused ARF induced F-actin cytoskeletal rearrangement in MC3T3-E1 cells. In addition, these cells showed an increase in intracellular calcium concentration following the application of focused ARF. Furthermore, passive bending movement was noted in primary cilium that were treated with focused ARF. Cell viability was not affected. Application of pulsed ultrasound radiation generated only a minimal temperature rise of 0.1°C, and induced a streaming resulting fluid shear stress of 0.186 dyne/cm(2), suggesting that hyperthermia and acoustic streaming might not be the main causes of the observed cell responses. In conclusion, these data provide more insight in the interactions between acoustic mechanical stress and osteoblastic cells. This experimental system could serve as basis for further exploration of the mechanosensing mechanism of osteoblasts triggered by ultrasound.
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