ArticlePloS one2012
RNA folding and catalysis mediated by iron (II).
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 89 citations in OpenAlex.
- Complex and Messy Prebiotic Chemistry: Obstacles and Opportunities for an RNA World.Life (Basel, Switzerland) · 2026Review
- Origin of ribonucleotide recognition motifs through ligand mimicry at early earth.RNA biology · 2024Review
- Investigation of tRNA-based relatedness within the Planctomycetes-Verrucomicrobia-Chlamydiae (PVC) superphylum: a comparative analysis.Archives of microbiology · 2023Article
- Goldilocks and RNA: where Mg2+ concentration is just right.Nucleic acids research · 2023Article
- Biological Catalysis and Information Storage Have Relied onBiomolecules · 2023Review
- How to build a protoribosome: structural insights from the first protoribosome constructs that have proven to be catalytically active.RNA (New York, N.Y.) · 2023Article
- Metals in Prebiotic Catalysis: A Possible Evolutionary Pathway for the Emergence of Metalloproteins.ACS omega · 2023Review
- Discovering riboswitches: the past and the future.Trends in biochemical sciences · 2023Review
- Iron-responsive riboswitches.Current opinion in chemical biology · 2022Review
- The Biochemical Landscape of Riboswitch Ligands.Biochemistry · 2022Article
- The Peptidyl Transferase Center: a Window to the Past.Microbiology and molecular biology reviews : MMBR · 2021Article
- Interchangeable utilization of metals: New perspectives on the impacts of metal ions employed in ancient and extant biomolecules.The Journal of biological chemistry · 2021Review
- In search of the RNA world on Mars.Geobiology · 2021Article
- Triphenilphosphonium Analogs of Chloramphenicol as Dual-Acting Antimicrobial and Antiproliferating Agents.Antibiotics (Basel, Switzerland) · 2021Article
- Oxidative Modifications of RNA and Its Potential Roles in Biosystem.Frontiers in molecular biosciences · 2021Review
- Oxidative Stress in Bacteria and the Central Dogma of Molecular Biology.Frontiers in molecular biosciences · 2021Review
- Iron-mediated degradation of ribosomes under oxidative stress is attenuated by manganese.The Journal of biological chemistry · 2020Article
- Prebiological Membranes and Their Role in the Emergence of Early Cellular Life.The Journal of membrane biology · 2020Review
- Cutting in-line with iron: ribosomal function and non-oxidative RNA cleavage.Nucleic acids research · 2020Article
- A noncanonical binding site of linezolid revealed via molecular dynamics simulations.Journal of computer-aided molecular design · 2020Article
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mg²⁺ shares a distinctive relationship with RNA, playing important and specific roles in the folding and function of essentially all large RNAs. Here we use theory and experiment to evaluate Fe²⁺ in the absence of free oxygen as a replacement for Mg²⁺ in RNA folding and catalysis. We describe both quantum mechanical calculations and experiments that suggest that the roles of Mg²⁺ in RNA folding and function can indeed be served by Fe²⁺. The results of quantum mechanical calculations show that the geometry of coordination of Fe²⁺ by RNA phosphates is similar to that of Mg²⁺. Chemical footprinting experiments suggest that the conformation of the Tetrahymena thermophila Group I intron P4-P6 domain RNA is conserved between complexes with Fe²⁺ or Mg²⁺. The catalytic activities of both the L1 ribozyme ligase, obtained previously by in vitro selection in the presence of Mg²⁺, and the hammerhead ribozyme are enhanced in the presence of Fe²⁺ compared to Mg²⁺. All chemical footprinting and ribozyme assays in the presence of Fe²⁺ were performed under anaerobic conditions. The primary motivation of this work is to understand RNA in plausible early earth conditions. Life originated during the early Archean Eon, characterized by a non-oxidative atmosphere and abundant soluble Fe²⁺. The combined biochemical and paleogeological data are consistent with a role for Fe²⁺ in an RNA World. RNA and Fe²⁺ could, in principle, support an array of RNA structures and catalytic functions more diverse than RNA with Mg²⁺ alone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.